儿童血清和脑脊液中NSE正常参考值的测定及其临床应用价值.pdfVIP

儿科药学杂志2004年第10卷第2期Journal ofPediatric Pharmacy 2004，Vo1．10，No．2 儿童血清和脑脊液中NSE正常参考值的测定及其临床应用价值 陈 琼，黄 志(重庆医科大学附属儿童医院，重庆 400014) [摘要]目的：确定正常健康儿童不同年龄之间血清和脑脊液中神经元特异性烯醇化酶(NSE)正常参考值。了解血清和脑脊液中 NSE之间的变化规律。探讨影响实验结果的各种因素以及血清和脑脊中NSE在新生儿HIE时的变化及其规律。方法：应用酶联免 疫法测定正常健康儿童和惠有HIE的新生儿血清和脑脊液中NSE水平。结果：66例正常健康儿童血清及脑脊液中NSE的水平．在 性别、年龄之间均无显著性差异(t=0．148、1．73，P均0．05)；影响血清和脑脊液中NSE检测结果的因素包括标本的采集方法、保存 及冻融、标本量及操作等；HIE患儿血清及脑脊液中NSE与病情轻重密切相关，即HIE越重，NSE升高越明显。轻、中、重三种类型 HIE三者分别相比，差异非常显著(t*2．95—6．38。P0．01)；健康儿童和HIE患儿血清NSE水平的变化与脑脊液中NSE水平的变化 成正相关(r=83．5—87-3)。结论：儿童由于血清和脑脊液中NSE水平没有性别及年龄差异，故只需设置一个正常参考值；检测血清中 NSE的改变情况可以反映大脑神经元受损的情况；检测血清和脑脊液中NSE水平变化可以帮助判断mE患儿病情的轻重．对指导 治疗及判断预后有重要的作用。 [关键词]神经元特异性烯醇化酶；缺氧缺血性脑病；正常值 [中圈分类号]R4J46．14 [文献标识码]A [文章编号]1672—108X(2004)02一O014-03 1be determination of normal value and clinical significance for NSE in serum and CSF in children CHEN Qiong，HUANG Zhi(Children’s Hospital,Chongqing University ofMedical Sciences，Chongqing 400014，Ch／n~) [Abstract]Objective：To define the reference range for Neuron—specific enolase(NSE)in serum and cerebmspinal fluid(cS among dif- ferent ages，and explore the relatoinship of NSE levels between in serum and in CSF among both healthy children and neonates witII HIE， meanwhile．to discuss various kinds of factors having influence on the result of the test．Methods：The NSE levels in serum and CSF were measured by ELISA in healthy children and neonates wi山 HIE．ResllIts： The NSE levels in seru,m and CSF didn’t correlated with sex and age in 66 healthy children．The specimen collection。preservation，dose of specimen and the process of operation were found as the factors influencing the test determination．The NSE levels in serunl and CSF in neonates wi山HIE correlated greatedly with the scvefity of 山e patient’S conchtion(仁2．95-．6．38，P0．01)．The changes of NSE le、rels in serum of bo山heathy children and neonates with HIE were very correlated to those in CSF(~--83．5-87．31．Conclusion：Sin