浙江汉族人群6个Y—STR基因座的遗传多态性调查及法医学应用.pdfVIP

· 技 术 交 流 · 刑事技术2003年第3期 浙江汉族人群 6个 Y—STR基因座的遗传多态性调 查及法医学应用 吴微微 ，郝宏蕾 ，郑小婷 ，张晓红 ，郑秀芬。 (1．浙江省公安厅刑侦总队，杭州310009；2．广东省广州市番禺区 公安分局，511400；3．公安部物证鉴定中心，北京 100038) 摘要： 目的 获得6个Y—STR基因座及其单倍型在浙江汉族人群中的遗传多态性分布，并探讨其法医学应用 价值。 方法 应用Y—plex荧光标记复合扩增系统，对浙江汉族2o0名无关男性个体进行6个STR基因座的复合 扩增，用ABI3100型基因分析仪对扩增产物进行检测，统计6个Y—STR基因座的群体遗传学参数。 结果 其中 5个Y—STR基因座分别检出5、7、6、6、5个等位基因，DYS385基因座检出47种单倍型，GD值最低为0．4275(DYS391)， 最高为0．9584(DY$385)；观察到6个Y—STR基因座共同构成的单倍型159种，其中有132种单倍型只出现1次，16 种出现2次，6种出现3次，2种出现4次，2种出现5次，累计GD值为0．9967。 结论 6个Y—sTR基因座具有较强的 个体识别能力，可应用于浙江法庭科学中的个体识别与亲权鉴定。 关键词： Y染色体；短串联重复序列(STR)；单倍型：遗传多态性 中图分类号：D918．2 文献标识码：A 文章编号： 1008—3650(2003)03—0016—08 Polymorphisms of six Y-chromosome STR loci in Han population in Zhejiang and its forensic appHcafion WU Wei-wei ，HAO Hong-lei，ZHENG Xiao—ting，et al(J．Department of Criminal Investigation， ， g Provincial Public Security Bureau，Hangzhou 310009,China) ABSTRACT： 0bjective To abtain the distribution of allele frequencies and haplotype of six Y-chromosome STR loci of Han population in Zhejiang，and to evaluate its forensic印plication．Method DYS393、DYS19、DYS389 1I、DYS390、DYS391、and DYS385 loci were amplified by using Y-plex PCR am plification Kit and were analyzed by 3100 genetic analyzer， 200 unrelated male individuals of Han population in Zhejiang were investigated to determine the distribution of allele frequencies and haplotype． Results 5、7、6．6、5 alleles were found in the preview 5 Y-STR loci， and 47 haplotypes were found in DYS385 locus． the minimum GD value WaS 0．4275(DYS391)， and the maximum was 0．9584(DYS385)． 159 haplotypes were found in 6 Y—STR loci， of which 132 were unique， 16 were found in 2 individuals， 6 were found in 3 individuals， 2 were found in 4 individuals， 2 were found in 5 individuals，and the observed haplotypes diversity value Was 0．9967． Conclusion The 6 Y—STR loci aye highly polymorphic and are suitable for forensic i