圆锥铁线莲醇提物治疗大鼠非细菌性前列腺炎的实验研究.PDFVIP

中国现代应用药学 2012 年 5 月第 29 卷第 5 期 Chin JMAP, 2012 May, Vol.29 No.5 ·381· 圆锥铁线莲醇提物治疗大鼠非细菌性前列腺炎的实验研究 刘小保 1,2，荣语媚 1，徐燕 1,2，李如意 1，张琳 1，张予阳 2*，田景奎 1*(1.浙江大学生物医学工程与仪器科学学院，教 育部生物医学工程重点实验室，杭州 310027；2.沈阳药科大学生命科学与生物制药学院，沈阳 110016) 摘要：目的 评价圆锥铁线莲醇提物对实验性大鼠急、慢性非细菌性前列腺炎的药效。方法 分别采用前列腺腹叶注入 角叉菜胶溶液和侧叶注入消痔灵注射液，建立大鼠急、慢性非细菌性前列腺炎模型，并以前列腺液中白细胞总数和卵磷 脂小体密度，前列腺指数，前列腺组织病理变化作为圆锥铁线莲醇提物的药效评价指标。结果 圆锥铁线莲醇提物呈剂 量依赖性的升高治疗组前列腺液中卵磷脂小体密度，降低前列腺指数和前列腺液中白细胞数，同时减轻病理切片中前列 腺组织炎症细胞浸润与损伤程度。结论 圆锥铁线莲对大鼠急、慢性非细菌性前列腺炎均有较好疗效。 关键词：圆锥铁线莲；非细菌性前列腺炎；角叉菜胶；消痔灵注射液 中图分类号：R281.4 文献标志码：A 文章编号：1007-7693(2012)05-0381-05 Experimental Study of Ethanol Extract of Clematis Terniflora DC. on Non-bacterial Prostatitis in Rats LIU Xiaobao1,2, RONG Yumei1, XU Yan1,2, LI Ruyi1, ZHANG Lin1, ZHANG Yuyang2*, TIAN Jingkui1*(1.Key Laboratory of Biomedical Engineering of Education, College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou 310027, China; 2.School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang 110016, China) ABSTRACT: OBJECTIVE To evaluate the effects of experimental treatment of the ethanol extract of Clematis terniflora DC(CTD) on the acute and chronic non-bacterial prostatitis(NBP) in rats. METHODS Use the methods of carrageenan and Xiaozhiling injection respectively into the ventral and lateral lobes of prostate to establish the acute and chronic non-bacterial prostatitis models in rats. The density of lecithin corpuscles(DLCC) and the number of leukocytes in prostatic fluid , the prostate index(PI) and the prostatic histopathological examination were used to evaluate the pharmacodynamics of the ethanol extract of CTD. RESULTS The CTD extract could dose-dependently increase the density of DLCC, decrease the PI and the number of leukocytes in prostatic fluid, also relieve the degree of the inflammational cell infiltration and lesion in tissues. CONCLUSION Clematis terniflo