基于差异蛋白质鉴别僵蚕及其新型伪品.pdfVIP

? 1024 ? 2014 年 10 月第 34 卷第 10 期 Jilin Joumal of Traditional Chi叩刷刷囚14 V，叫No.10 毒待命叫 以)1: 10.1到63/j.c地i. j均y.2014. 1O .019 基于差异蛋白质鉴别僵蚕及其新型伪品 邱 乙，汪云伟，程元柳，吴纯洁，万德光，国锦琳椅 (成都中医药大学中药材标准化教育部重点实验室 中药资源系统研究与开发利用省部共建国家重点实验室培育基地，成都 611 137) 摘要:目的 通过总蛋白含量、可溶性蛋白含量差异以及应用 SDS蛋白质电泳寻找差异蛋白质来鉴 别僵蚕与伪品黑死蚕、空心僵蚕。方法 采用 SDS法对僵蚕真品与伪品差异蛋白条带进行鉴别。结果 发现僵蚕总蛋白含量不低于 60.∞%，可溶性蛋白含量不低于 4.29% ，伪品远低于上述指标，可作为僵蚕质 量评价的检查项指标，辅助鉴别僵蚕与其伪品;僵蚕特有差异蛋白质 (SP) 的有无和含量差异，可有效鉴别上述 两种新型伪品。结论 本研究初步建立基于差异蛋白质的僵蚕真伪鉴别新方法，为下一步完善僵蚕质量标准， 以及僵蚕的质量控制提供科学的依据。 关键词:僵蚕;新型伪品 ;SDS; 差异蛋白质 中图分类号: R282.5 文献标志码 :A 文章编号: 1∞3 - 5699(2014) 10 - 1024 - 03 Pre恤回nary s阳dy on identification of bombyx batryticatus and new adulterants using differential proteins QIU Yi , WANG Yunwei , CHENG Yu缸ùiu ， WU Chunjie , WAN De伊ang ， GUO Jinlin 祷 (Chengdu University of Traditional Chinese Medici时， Key Laboratory of Standardization of Chinese Medicine of Ministry of Education , State Key Laboratory Breeding Base of Research and Development of Chinese Medicinal Re鸣。urces ， Chengdu 611137 , China) Abstract: Obj配tive The characteristic proteins were found 出rough difference of to凶 protein contents and soluble protein contents and SDSprotein battery to identify Bombyx Batryticatus (Jiang can) and its adulterants. Me由ed Using SDSme出od to identi马r differences protein bands from silkworm genuine and its counterfeit goods. R臼u1t Total pro- tein content exceed 60.0% and solubility protein content exceed 4.290毛 in Bombyx Ba问吨icatus (Jiang c咽)， which bo由 outclass adulterants above-mentioned , this can be used as an effective index for examination of the quality evaluation of Bom- byx Batryticatus , and assist to insure its identity. The existence and different content of the special characteristic protein (SP) in Bombyx Batrγticatus can be used for identification of the two new adulterants above-mentioned. Conclusion 咀lis analy- sis preliminarily established new methods of Bombyx Batrytic现tus identification based on the stable difference of protein , and laid 出e