左向右分流容量负荷过重大鼠血红素氧化酶的表达及氯沙坦对其的影响.pdfVIP

左向右分流容量负荷过重大鼠血红素氧化酶的 表达及氯沙坦对其的影响 袁粒星 刘瀚旻A 李 谧 高 举 周同甫 四川大学华西第二医院 儿科 ( 成都 610041) [ 摘要 ) 目的 探讨容量负荷过重大鼠及氯沙坦治疗后肺动脉高压肺血管重建与血红素氧化酶 ( HO ) mRNA 表达水平的关系 O 方法 行左向右分流术建立容量负荷过重 SD 大鼠模型并给予氯沙坦治疗 分别测定实验各组肺 动脉压 ~ 右心室压等血流动力学指标 ; 计算右心室相对重量 ; 体视学方法测定肺小动脉厚度百分比和肌性化小动脉 百分比 ; 逆转录多聚酶链反应 ( RT - PCR ) 测定大鼠肺组织 ~ 左右心室 HO -1~ HO -2 mRNA 的表达 O 结果 氯沙坦能 显著减低肺动脉压力 使右心室相对重量降低 氯沙坦组肺小动脉厚度百分比和肌性化小动脉百分比均较手术组 明显下降 O 手术组 HO -1 mRNA 表达较对照组显著增高 而氯沙坦组 HO -1 mRNA 表达水平较手术组明显降低 HO -2 mRNA 表达在三组间差异无统计学意义 O 结论 氯沙坦能显著降低容量负荷过重大鼠的肺动脉高压 抑制 肺血管重构程度 同时可下调 HO -1 mRNA 的表达 O HO -1 参与了肺动脉高压形成 ~ 肺血管重构的病理过程 O [ 关键词 ) 容量负荷 氯沙坦 血红素氧化酶 肺血管重建 [ 中图分类号 ) R 72s. 4 Ef f ects of Losartan on Expression of Heme Oxygenases in Volume - overloaded Rats with Lef t - to - right Shunt YUAN Li - xing LIU Hon - min A LI mi GAO Ju ZHOU Tong - fu . DePa7 ?en 0f PeCla 7lcS WeS Chlna Sec0nC H0SPl aZ Slchuan UnlUe7Sl y Chengdu 610041 China [ Abstract ) Objective To study the expression of heme oxygenase -1 mRNA and pulmonary vascular remodeling before and af ter surgical establishment of lef t - to - r ight shunt in volume - overloaded SD rats and in SD rats With Losartan intervention . Methods Lef t - to - r ight shunt volume - overloaded SD rat models Were established by aortocaval shunt operation . Seven rats With shunt Were placed on Losartan ( Losartan group ) 7 rats With shunt but not given Losartan Were included in the operation group and 4 rats af ter sham operation served as controls . Pulmonary pressure and right ventricular pressure Were measured during catheterization . The relative Weights of ventricles Were determined af ter execution of the rats . Pulmonary vascular remodeling parameters including percentage arterial Wall thickness and percentage muscularized small arteries Were assessed by morphometry . Heme oxygenase -1 ( HO -1) mRNA expression and heme oxygenase -2 ( HO -2) mRNA expression Were detected by RT - PCR method . Results Pulmonary artery pressure and right ventricular relat