结核分枝杆菌抗体双抗原夹心ELISA检测方法的建立及初步应用.pdfVIP

doi??10??3969??j??issn??1009??0002??2015??05??021 研究报告 ??????????????????????????ELISA???????????????? 初步应用 ??????1??2????????3????????4????????1????????1??????5?? ??????1????????1??????1????????1??????2????????1????????1 1????????????????????????????????????100850??2????????????????????????I????．华北石油总医院，河 北任丘???：??绶??胖肮ひ皆海?颖北６?????．首都师范大学数学科学学院，北京??? ?R猐 目??：建立?纸岷朔种Ω司???抗体的双抗原夹心???觳夥椒ǎ?⒊醪狡兰燮湓贛?血清学临床辅 助诊断中的应用价值：方法：采用??诤峡乖?????????魑0?豢乖??直鹨岳备??趸?锩??? 和生物素???昙堑?????????魑1昙强乖????种??n?乖?行腅??法，即??狤??法和 ??狤??法；采用所建立的?址椒ǘ越岷嘶颊吆徒】刀哉昭G褰?屑觳?结果：经过一系列的反应条件优化，确 定??狤??法和??狤??法的最佳抗原包被浓度分别为??．? ??痬??钍始友?烤??? txL???????????? ??????????????????????l??500??l??2000????????????????????43??66????52??11??????????????100????????????????2 ??Mtb??????????ELISA????????????????Mtb?????????????????????? ?丶?蔧 ??????????????????????38kD+ESAT6+CFPl0????????????ELISA ?型挤掷嗪臸??籖??? [??????????]A?恼卤嗪臸 ??—?????????? Establishment ? ??? Antigen ???? ELISA and Their ??? ??? ? Detecting ????? for Mycobacterium ?????? JIANG Shu??Fang??2??GUOLan??Qin3??ZONGlie??Lian94??YANGXi??Qin???L|U??猀??：，?? ???瑇? Bing??ShuiI??DUAN??狹?瑉?? xu-Huii??CHENKun|??ZHANGRon92????FENG Xiao??Yahi????ZHANG?—?? ?甀???? ? Basic Medical Sciences??Academy? ???? Medical Sciences??Beijing100850??2??ShenyangUniver- ?? ? ????．???? 1 10016??3??General???? ? ??? Petroleum??Renqiu062552??4??Worker7sHospi?? tal ? ?? ???珺??? ? 1 ???．??? ? ?????? Sciences??Capital ??? University??Beijing 1