叶片位置和切除方式对巨细桉DH201-2再生的影响-广东林业科技.doc

叶片位置和切除方式对巨细桉DH201-2再生的影响基金项目：国家“863” “白桦、桉树等分子育种与品种创制”（2011AA100202）、热林所基本科研项目“桉树栽培无性系转基因技术与转基因育种研究”（2007-23） 作者简介：刘英（1963—），女，硕士，高级实验师，主要从事林木组培快繁及植株再生等研究。 *通讯作者:曾炳山（1967-），男，女，博士，研究员，主要从事林木组培快繁与转基因研究。 刘英 曾炳山＊　裘珍飞　李湘阳 （中国林业科学研究院热带林业研究所，广东　广州　510520） 摘要：以巨细桉无性系DH201-2叶片为材料进行植株再生研究，揭示叶片位置和切除方式对愈伤组织诱导和植株再生的影响，结果表明：叶片位置对叶片培养死亡率与叶片植株再生率、叶片植株再生芽数影响显著对愈伤产生率基本没影响，以组培生根苗第1-6片叶作外植体进行愈伤培养与植株再生培养效果较好，以第1-2片叶作外植体效果最佳，第7-8片叶不宜作为外植体培养；拉下或剪下全叶两种方式，植株再生率与再生芽数较高，全叶剪除1/3叶尖方式效果最佳，能有效加速叶片的愈伤生长及植株分化，提高叶片植株再生率及再生芽数。全叶剪除叶柄及1/3叶尖方式效果最差，植株再生率、再生芽数效果最低。培养50天，愈伤植株再生率及再生芽数达到高峰期。 关键词：巨细桉　外植体选择　预处理　植株再生　 中图分类号：　　　　文献标识码：A Effect of leaf position and Pretreatment on the Regeneration of an Eucalyptus.grandis ×E. tereticornis clone LIU Ying ZENG Bing-shan* QIU Zhen-fei LI Xiang-yang (The Research Institute of Tropical Forestry, CAF, Guangdong Guangzhou 510520) Abstract This paper deals with the regeneration of leaf explants of an E.grandis ×E.tereticornis clone DH201-2. Different leaf position shows basically no influence on callus induction but eminent effect on death rate, regeneration rate and shoot number of leaf explants. The first leaf to the sixth leaf numbered from the top of rooted planlets are most suitable explants for regeneration,especially for the first two leaves. The seventh leaf and eighth leaf are not competent for regeneration; Pulling down or cutting the whole leaf can get high regeneration rate and shoot number. Excision of one third top part of leaf explants is the best pretreatment. Callus formation speed, regeneration speed, regeneration rate and shoot number are increased by this pretreatment. Excision of two thirds top part of leaf explants will get a poor result,;Excision of petiole and one third top part of leaf explants is the worst pretreatment with the minimum quantity of the regeneration rate and the shoot number. The regeneration rate was the best when the callus was 50 days. Key words: E.grandis ×E.tereticornis; Selection of explant; Pretreatment; Plantlet re