215 cases of blood neutrophils in patients with the results of ALP activity_0.docVIP

 PAGE \* MERGEFORMAT 6 215 cases of blood neutrophils in patients with the results of ALP activity [Abstract] Objective To study the neutrophil alkaline phosphatase (NAP activity was measured in the blood differential diagnosis of disease. Methods 215 cases of blood collected patients and 30 healthy human venous blood smear preparation, even by Kaplow nitrogen coupling method NAP staining, oil immersion microscope observation of 100 mature neutrophils to five integral record the results. Results of acute lymphoblastic leukemia (ALL), leukemoid reaction (LR), aplastic anemia (AA), chronic lymphocytic leukemia (CLL), multiple myeloma (MM), polycythemia vera (PV) 6 group of diseases was significantly higher NAP points (P lt;0.01), CLL, malignant histiocytosis (MH), myelodysplastic syndrome (MDS-RAEBT), paroxysmal nocturnal hemoglobinuria (PNH) 4 group of diseases was significantly lower than the 6 points NAP group of diseases and the control group (P lt;0.01), AML NAP Integration slightly lower than the control group, but the difference was not significant (Pgt; 0.05), and ALL the difference was highly significant (P lt;0.01). Conclusion The NAP activity assay can support the differential diagnosis of blood diseases. [Keywords:] alkaline phosphatase, blood disease Alkaline phosphatase were mainly seen in mature neutrophils, reticular cells and macrophages, other cells are all negative. Department of Hematology, 215 cases in our hospital the patient’s blood neutrophil alkaline phosphatase (NAP) activity observed comparative analysis to explore the NAP in the differential diagnosis of blood diseases in the application. 1 Materials and Methods 1.1 General information on 215 cases of blood diseases in this group are from the Department of Hematology in our hospital from 2002 to 2007 analysis by the bone marrow smear cytology, pathology and related laboratory and clinical reference [1] confirmed cases, including aplastic anemia (AA) 47 cases of myelodysp