Allograft activated host dendritic cells-mediated immune tolerance.docVIP

 PAGE \* MERGEFORMAT 19 Allograft activated host dendritic cells-mediated immune tolerance Author: Huetsch Qi, Li Mei, Wang Songxia, Xue Yan Tong [Abstract] Objective To develop a mechanism based on the theory T cells to induce graft special antigen for immune tolerance of the opposite sex. Method first used lethal dose of γ -rays, will be subject to those T-cells and stem cells removed, forming a non-stem cells, pre-T cells and T cells in the recipient model, claiming that the recipient model Ⅰ . At the same time through the same kinds of manufacture of the normal skin graft on the donor organ recipient’s immune rejection of the model, said model Ⅱ . From the model Ⅱ and obtained by magnetic activated cell sorting were activated by donor antigens, with antigen-presenting function of dendritic cells, and normal pre-prepared recipient bone marrow cells in vitro to a mixture of transfusion recipients in the model Ⅰ . Ⅰ recipient hematopoietic function reconstruction after the completion of immunization, and then its OK with the kinds of skin graft. Divided into 5 groups were used to observe the survival time of skin grafts, and routine pathological analysis of tissue biopsies and flow cytometry detection. The results from the upper reaches of T cell development induced specific immune tolerance to transplant, comparing the control group, experimental group, skin graft survival time was significantly prolonged (P amp;lt;0.005), flow cytometry and histopathological analysis of differences are also significant. Conclusion The established models and systems, can induce graft-specific immune tolerance, but also further validation of the thymus in negative selection theory. [Keywords:] dendritic cell; transplantation; tolerance; thymus; T cell development Induction of transplantation tolerance with allograft-activated host dendritic cells [Abstract] Objective The negative thymic selection is critical for the maturation of bone marrow derived cells to T cel