Amniotic fluid cell culture on the efficiency improvement in the clinical application of prenatal diagnosis.docVIP

 PAGE \* MERGEFORMAT 7 Amniotic fluid cell culture on the efficiency improvement in the clinical application of prenatal diagnosis [Key Words] high amniotic fluid cell culture improved the clinical application of prenatal diagnosis Prenatal diagnosis of amniotic fluid cell culture is an important technical means, mainly used in prenatal diagnosis of fetal chromosomal diseases. Since relatively few surviving cells in amniotic fluid, cultured cycle is long, sterile and technical requirements, easy to fail, so that amniotic fluid cells training in the application of prenatal diagnosis is limited. For the above, in recent years we have learned some of the advanced experience of home and abroad [1,2], with its own conditions, to cooperate with relevant departments, on the amniotic fluid cell culture technology is improved and 1 200 cases of pregnant women 15 to 30 weeks were cultured amniotic fluid cells for prenatal diagnosis, achieved the desired results. Subjects and methods 1. PARTICIPANTS: July 2003 to December 2005 in Xinxiang City, Henan Province, MCH antenatal check the amniotic fluid cell cultures a total of 1 200 pregnant women treated with normal amniotic fluid cell culture (conventional group) and the efficient improvement of amniotic fluid cell culture (improvement group) were taken from 600 patients. to patient ratio of 1:1 of pregnant women were randomly divided into 2 groups. age distribution of the conventional group (28.8 + -3.6 years), gestational age distribution of 15 to 24 weeks gestation, the average (20.2 + -2.8 ) weeks, the age distribution improvement group (28.5 + -3.7) years, 15 to 24 weeks gestational age distribution, the average (20.3 + -2.7) weeks, 2 groups of age, gestational age, the difference was not significant (Pgt; 0.05) . The efficiency improvement of scientific research through the amniotic fluid cell cultures and Child Health Hospital of Xinxiang Medical Ethics Committee approval and implementation. 2. Testing