Chinese Naoxintong on endothelial nitric oxide and endothelin1 of.docVIP

 PAGE \* MERGEFORMAT 15 Chinese Naoxintong on endothelial nitric oxide and endothelin1 of Of: Liu Shengqiang, Wang Dongqi, Leixin Jun, Chang-Cong Cui, Li Hongbing, Liu Qian [Abstract] Objective To observe Naoxintong on oxidized low density lipoprotein (Ox LDL) induced human umbilical vein endothelial cells (HUVEC) nitric oxide (NO and endothelin 1 (ET 1 secretion. Methods to foster HUVEC as target cells, endothelial cells in the medium with Ox LDL (50 mg / L Preparation of cell injury, and containing different concentrations of serum Naoxintong pre-intervention, respectively, and by nitrate reductase method cell supernatant were measured by radioimmunoassay The content of NO and ET 1. Results Ox LDL effect HUVEC 24 h after, HUVEC supernatant NO content decreased, while the ET 1 were significantly increased, were significantly higher than the control group (P lt;0.05, and pre-accession Naoxintong containing serum or liquid atorvastatin group was significantly higher NO levels, and in the added step Naoxintong containing serum group, this effect with a certain dose range increased with the increase of drug dose (P lt;0.05. Conclusion Naoxintong can step through the promotion of endothelial cell NO synthesis and release of vascular endothelial cells and thus play a protective role, will help reduce or inhibit the formation of atherosclerosis and development. [Keywords:] Naoxintong, vascular endothelial cells, oxidized low density lipoprotein, nitric oxide, endothelin-1 Abstract: ObjectiveTo investigate the effect of NaoXinTong (NXT) on the release of nitric oxide (NO) and endothelin-1 (ET-1) of human umbilicalvascular endothelial cells (HUVEC) from injury induced by Ox-LDL and to study the therapeutic mechanism of NXT in treating atherosclerosis (AS). MethodsOx-LDL of 50mg / L was used co-incubated with HUVECs for 24h in vitro to establish injury model of HUVEC and NXT containing serum was applied to implement intervention 12 hours ahead of Ox-LDL. The