CIK cells against leukemia effect of minimal residual disease.doc

 PAGE \* MERGEFORMAT 12 CIK cells against leukemia effect of minimal residual disease Of: Xiao Zhongping Feng Tao Wang Mei Han Xiao Zhongping Xueye [Abstract] Objective To observe the CIK cells against autologous, allogeneic primary leukemia cell killing effect on the G0 of CD34 + leukemic cells, cytotoxicity, and the normal bone marrow hematopoietic stem cells. Methods of acute leukemia (AL) after chemotherapy, chronic myeloid leukemia (CML) in chronic phase and normal peripheral blood mononuclear cells cultured by adding a series of cytokines induced by CIK cells, CIK phenotype by flow cytometry, G CIK with karyotype analysis. cryopreservation, as the target recovery cells, MTT method CIK cells against autologous, allogeneic primary leukemia cell killing effect. AO staining method recovery G0 of acute myeloid leukemia (AML) CD34 + cell ratio and cultured CML CD34 + cells in the G0 phase cells. Flow cells detected before and after CIK cell killing G0 CD34 + cell percentage of change. semi-solid culture CIK cells were detected in normal bone marrow CFU GM, BFU E colony formation. The results CIK cells obtained from peripheral blood of leukemia patients, derived from normal lymph cells against autologous primary leukemia cells was killing effect on the G0 leukemia cells inhibited, the normal hematopoietic stem cells without killing effect. Conclusion CIK cells can be used to remove residual leukemia cells an effective tool. [Keywords:] leukemia, minimal residual disease, CIK cells Complete remission of the leukemia patients in vivo, often remnants of resistance, minimal residual leukemia in quiescent cells, is the source of relapse. In this study, cultured from the peripheral blood of leukemia patients out of the CIK cells do not contain leukemia stem cells, can effectively kill G0 of CD34 + leukemic stem cells, and this selective killing effect, no damage to normal hematopoietic stem cells, immune cells can be used as an effective body to remove leu