Cockroach herbs Quality Control Method.docVIP

 PAGE \* MERGEFORMAT 13 Cockroach herbs Quality Control Method Authors: Yang Yongshou, XIAO Pei-yun, LIU Guang-ming [Abstract] Objective To establish the quality control of medicinal cockroach. Official Methods of thin-layer chromatography identification, chromatographic conditions were as n-butanol - acetic acid - water (5:1:1), 0.5% ninhydrin reagent; determined using Soxhlet extraction of fat content; Moisture Determination of Moisture Inspection The minimum control limits; spectrophotometric determination of total amino acid content of ninhydrin color after the detection wavelength was 570 nm. Results Cockroach medicines and reference substance TLC spots were clear and easy to identify, medicinal herbs in the fat content should not exceed 7.0%, moisture content should not exceed 12.0%, spectrophotometric determination of total amino acid content, the regression equation: A = 0.067 943C 0.009 143, r = 0.999 9, tyrosine content of 1.0 ~ 10.0 μ g was well within the linear relationship. Conclusion The method is simple, reproducible and can be used for quality control of medicines cockroach. [Keywords:] Cockroach medicine TLC Determination of water Soxhlet extraction spectrophotometry Abstract: ObjectiveTo establish the quality control of Roach. MethodsTLC was used to identify the quality of Roach. The chromatographic condition was n-butanol-HAc-H2O (5:1:1), and the developer was triketohydrindene hydrate (0.5%); Soxhlet’s extraction was used for the determination of fat; moisture content was detected by the Aquametry; the content of amino acids was determined by Absorption spectrometry, the developer was triketohydrindene hydrate, and the warvlength was 570 nm.ResultsRoach spots and control article spots were very clear by TLC identification. The fat content of Roach was less than 8.0%, and the Moisture content was less than 12.0%. The amino acids were measured by absorption spectrometry and the regression equation was A = 0.067 943C 0.009 143, (r = 0.999