CTGF antisense oligonucleotide on Ang Ⅱ -induced human proximal tubular epithelial cells of.docVIP

 PAGE \* MERGEFORMAT 6 CTGF antisense oligonucleotide on Ang Ⅱ -induced human proximal tubular epithelial cells of Author: Chen Long, Liu Bi-Cheng, MA Kun-Ling, Liu Hong, LUO Dong-dong Objective: To explore the connective tissue growth factor (CTGF) antisense oligonucleotide on angiotensin Ⅱ (Ang Ⅱ )-induced transdifferentiation of renal tubular cells, the possible effects. Methods: Cultured human proximal tubular epithelial cell line (HK2), were observed CTGF antisense oligodeoxynucleotide, Ang Ⅱ -cell interference on cell CTGF mRNA and protein expression, cell ultrastructure and cell α -smooth muscle actin (α SMA) expression. Results: Ang Ⅱ intervention in HK2 cells, after 48h, cell expression of CTGF mRNA and protein were significantly higher (P amp;lt;0.01); Ang Ⅱ intervention 96h, the cellular ultrastructure showed mesenchymal cell-like structure; these effects can all be CTGF antisense oligonucleotide nucleotides significantly inhibited. Ang Ⅱ can be a time-dependent manner stimulated HK2 cells expressed α SMA, such effects can be CTGF antisense oligonucleotide significantly inhibited (P amp;lt;0.01). Control group, missense oligonucleotides had no such effect. Conclusion: CTGF antisense oligonucleotide on Ang Ⅱ -induced transdifferentiation of HK2 cells significantly inhibited, suggesting that CTGF might be mediated by Ang Ⅱ -induced transdifferentiation of renal tubular epithelial cells. [Keywords:] human proximal tubular epithelial cells; angiotensin Ⅱ ; connective tissue growth factor; cell transdifferentiation Abstract: Objective To investigate the effect of connective tissue growth factor antisense oligonucleotide (CTGF AS) on Ang Ⅱ induced tubular cell transdifferentiation. Methods The influence of CTGF AS on Ang Ⅱ induced HK2 cells CTGF mRNA and protein expression were observed by RTPCR and confocal microscopy respectively. The effect of CTGF AS on Ang Ⅱ induced cellular ultrastructure were observed by transmissive electronic microscope