Daqingye indirubin in Supercritical Fluid Extraction Technology and Determination.docVIP

 PAGE \* MERGEFORMAT 12 Daqingye indirubin in Supercritical Fluid Extraction Technology and Determination 【Key Words】 Daqingye SFE technology of indirubin from Daqingye and content determination of indirubin 【Abstract】 AIM: To use HPLC for the content determination of indirubin in supercritical fluid extraction (SFE) material from Daqingye and to optimize the process of SFE. METHODS: Content of indirubin was used as index to evaluate the technologies based on orthogonal design, in which 4 factors considered were extraction temperature and pressure, separation temperature and pressure. A HPLC system was used to determine the content of indirubin. RESULTS: Optimal program was as follows: extraction temperature 50 ℃ and pressure 40 MPa, separation temperature 40 ℃ and pressure 8 MPa. The average recovery of indirubin was 102.8% and RSD was 1.48%. CONCLUSION: The extraction temperature, extraction pressure and separation pressure all have significant effects on the extraction rate of indirubin. The HPLC method is simple, precise and suitable for the content determination of indirubin in SFE material of Daqingye. 【Keywords】 Daqingye; SFECO2; indirubin; chromatography, high pressure liquid 【Abstract】 Objective: To establish HPLC determination of Daqingye supercritical extract of indirubin content method, the extraction process were optimized. Methods: supercritical fluid extraction method L9 (34) orthogonal experiment. To indigo-yu Red content inspection extraction temperature, extraction pressure, separation temperature and pressure of four separate factors. using HPLC determination of indirubin content of extracts. Results: The optimum extraction process for the extraction temperature 50 ℃, extraction pressure 40 MPa, separation of temperature of 40 ℃ and the separation pressure 8 MPa. indirubin average recovery was 102.8%, RSD 1.48%. Conclusion: The extraction temperature, extraction pressure and separation of pressure on the extrac