DNA identification of molecular markers in the application of traditional Chinese medicine and development trend.docVIP

 PAGE \* MERGEFORMAT 24 DNA identification of molecular markers in the application of traditional Chinese medicine and development trend Of: Li Li, Xu Yongli, Zhang Yueyun, Zhao Chengjian [Abstract] Objective of the DNA molecular markers identified in the field of traditional Chinese medicine reviews the application. Methods inspection since the 20th century, 90 years to summarize the published literature. Results of molecular genetic markers with DNA trace, rapid, specific and , accurate and reliable, less demanding on the characteristics of the sample, using more methods are RAPD, ISSR, ITS markers and rRNA gene sequence analysis techniques. At the same time, DNA molecular markers also exist with limitations on the experimental hardware and practitioners The technically demanding, high cost, technical complexity and high medicinal herbs on different parts of the still difficult to distinguish, and can not be used for medicine, compound, extract identified weaknesses. Conclusions DNA molecular markers as a method of Chinese herbal medicines have yet to be identified development and improvement. [Keywords:] DNA molecular markers, Chinese herbal medicine, identification Abstract: ObjectiveThe paper summarizes the researches of application of DNA molecular markers in Chinese medicinal materia.MethodsInduction and analysis were used by referring to literature released since 1990s. ResultsDNA Marker Technologies have characteristics of trace, rapid, peculiarity, accuracy and credibility, lower requirements for the sample. Random amplified polymorphic DNA (RAPD), inter-simple sequence repeat (ISSR), interial transcribed spaoers (ITS) and rRNA gene sequencing technology are often used.However, DNA Marker Technologies have some disadvantages that employees are required special skills, the cost is high and the technique is complicated, while it is difficult to distinguish different parts of Chinese medicinal materia used medicinally and identify patent medi