Drosophila S2 cells high expression of luciferase activity of recombinant plasmid and determination of.docVIP

 PAGE \* MERGEFORMAT 5 Drosophila S2 cells high expression of luciferase activity of recombinant plasmid and determination of [Abstract] Objective: To construct highly expressed in Drosophila S2 cells, recombinant luciferase plasmid. Methods: The PCR method amplified pac gene promoter by restriction enzyme digestion was subcloned into the eukaryotic expression vector pGL3Basic in transfected Drosophila S2 cells, by measuring luciferase and β -galactosidase activity in the calculation The relative luciferase activity. Results: The constructed recombinant plasmid in S2 cells, the relative luciferase activity compared with pGL3Control in an increase of 2.7 million times. Conclusion: The successful construction of the Drosophila S2 cells, high expression of luciferase plasmid pGL3pac. [Keywords:] Drosophila S2 cells; luciferase; promoter; reporter gene; transfection In the study of gene function in the process will inevitably have to study gene regulation mechanisms, and gene expression product of complex and difficult for its quantitative and qualitative, to cell cycle at any point of understanding of cell gene expression product is very difficult to . However, the report found that genetic analysis system to study gene regulation and expression has brought new hope. In recent years, the study reporter gene system has achieved rapid development. Reporter gene is an easy-to-detect encoding a protein or enzyme gene expression through its product to calibrate the target gene expression regulation, therefore, the understanding of eukaryotic gene regulation can be measured not by direct transcriptional regulatory elements regulating the ability to rate , but the cis-regulatory elements and the reporter gene sequence to link the process of gene transcription in the determination of reporter gene expression levels of transcripts in order to determine the corresponding cis-acting elements of the regulatory capacity. As a reporter gene must have the following characteri