食管鳞癌组织中Livin和Smac表达的论文.docVIP

　　食管鳞癌组织中Livin和Smac表达的论文 【摘要】 目的探讨食管鳞癌组织中凋亡抑制蛋白livin和促凋亡基因smac蛋白的表达及其与食管鳞癌临床生物学指标的关系。方法应用免疫组化sp法对62例食管鳞癌组织、31例癌旁不典型增生组织及62例正常食管黏膜组织进行livin和smac蛋白的检测，分析其阳性表达与食管鳞癌患者临床病理因素的关系。结果62例食管鳞癌组织中，组织学分级ⅰ级15例、ⅱ级25例、ⅲ级22例；有淋巴结转移20例；肿瘤浸润至黏膜层、黏膜下层或浅肌层7例、深肌层14例、纤维膜41例。livin蛋白在正常食管黏膜组织中低表达，在癌旁不典型增生组织及食管鳞癌组织中高表达，阳性率分别为38.7%（24/62）、61.3%（19/31）和80.6%（50/62），差异有统计学意义（χ2=22.742，plt;0.05）；smac蛋白在癌旁不典型增生组织中高表达，在正常食管黏膜组织及食管鳞癌组织中低表达，阳性率分别为67.7%（21/31）、48.4%（30/62）和33.9%（21/62），差异也有统计学意义（χ2=9.688，plt;0.05）。食管鳞癌患者癌组织中livin和smac蛋白的表达与性别、年龄无关；与组织学分级、浸润深度及有无淋巴结转移有关（plt;0.05）。livin和smac蛋白在食管鳞癌组织中的表达呈负相关（r=－0.426，plt;0.05）。结论livin和smac在食管鳞癌组织中呈现异常表达，提示两者参与了食管鳞癌的发生发展和浸润转移。 【关键词】 食管鳞癌；凋亡抑制蛋白livin；促凋亡基因smac；免疫组织化学 zhao qiumin1,2 1. department of pathology, the first affiliated hospital, zhengzhou university, henan key laboratory of tumor pathology, zhengzhou 450052, china;2.editorial board of journal of zhengzhou university(medical sciences)abstract：objective to investigate the expression of livin and smac proteins in esophageal squamous cell carcinoma (escc) tissue and their relationship to the progression of escc.methodsexpressions of livin and smac proteins munohistochemistry sp method in 62 cases of escc, in 31 cases of paracancerous atypical hyperplasia, and in 62 cases of normal esophageal mucosa. then the relationships betac protein expressions and clinicopathologic features of escc patients ated.resultsof the 62 escc patients, ther diagnosis of escc ed by histopathological detection, 15 cases ph node metastasis; and 7 cases ucosa, submucosa, or shallouscle, 41 cases have invasion of fiber membrane. livin protein al esophageal mucosa, but high expressed in paracancerous dysplasia and esophageal cancer tissues, and the difference among the 3 groups ac protein al esophageal mucosa and esophageal cancer tissues, and the difference among the 3 groups ac protein expressions in escc tissues ph node metastases (plt;0.05). the expression of livin and smac proteins al expression of livin and smac o