Effects of inosine on mice model of Parkinsons disease neuroprotection.docVIP

 PAGE \* MERGEFORMAT 6 Effects of inosine on mice model of Parkinson’s disease neuroprotection [Keywords:] mice Inosine is neuroprective against MPTPinduced Parkinsons disease in C57BL mice [Abstract] AIM: To study the neuroprotective effects of inosine on 1methyl4phenyl1, 2,3,6 tetrahydropyridine (MPTP) induced Parkinsons disease (PD) in C57BL mice. METHODS: The PD models were formed with intraperitoneal injections of MPTP. Inosine was administered intraperitoneally to mice 30 min prior to MPTP. The effects of inosine and MPTP on the behavioral exhibition, the number of dopamine (DA) neurons in the substantia nigra and the density of tyrosine hydroxylaseimmunoreactive (THir) nerve fibers in the striatum, and the level of DA in the striatum were studied by behavioral test, immunohistochemical analysis and fluorospectrophotometry. RESULTS: The scores of locomotion, Rotarod and swim test decreased by about 45% and 43% and 22%, respectively. The number of DA neurons in the substantia nigra declined by about 58%, the density of THir nerve fibers also decreased, and the level of DA in the striatum declined by about 88%. Inosine, given prior to MPTP, attenuated the effects of MPTP. The scores of locomotion, Rotarod and swim test declined only by about 5% and 11% and 12%, respectively, the number of DA neurons in the substantia nigra declined by only about 43% and the level of DA in the striatum declined only by about 71%. CONCLUSION: Inosine is neuroprective against MPTPinduced lesions in C57BL mice. [Keywords] inosine; 1Methyl4pheny1, 2,3,6 tetrahydropyridine; Parkinson disease; nearaprotective agents; mice [Abstract] Objective: To observe the inosine on MPTP-induced Parkinson’s disease (PD) neuroprotective effect of a mouse model. Methods: C57BL mice with MPTP established PD model, given before MPTP inosine, by behavior detection (autonomous activity count, Rotarod test, swimming test), immunohistochemistry and fluorescence spectrophotometry, observed a mouse