EGCG on high glucose-induced rat mesangial cell proliferation and regulation of inflammatory cytokines.docVIP

 PAGE \* MERGEFORMAT 7 EGCG on high glucose-induced rat mesangial cell proliferation and regulation of inflammatory cytokines [Abstract] Objective To study the table epigallocatechin gallate (EGCG) on high glucose-induced rat mesangial cell proliferation and inflammatory cytokines (MCP  1 and ICAM  1) secretion. Methods cultured rat mesangial cells for research. The establishment of low-sugar control group (NG) and high glucose group (HG, E100, E200, E400, respectively, with the EGCG 0,100,200,400 μ g * L-1), were cultured 12,24,48 h. CCK cell count to detect different concentrations of EGCG in rat mesangial cells under the action of the OD values, to determine cell proliferation. Using ELISA, cell culture supernatant MCP  1 and ICAM  1 protein concentration. Results EGCG could inhibit high glucose mesangial cell proliferation activity and its inhibition in a dose-and time-dependent manner. Mesangial cells in high glucose environment, MCP  1 and ICAM  1 protein expression, low concentrations of EGCG on the MCP  1 and ICAM  1 protein in the inhibitory effect was not obvious, high concentrations of EGCG significantly inhibited the MCP  1 and ICAM  1 secretion. Conclusion In vitro high glucose conditions, the rat mesangial cells in the proliferation within 48h mainly a certain concentration of EGCG inhibited the proliferation. EGCG can inhibit protein levels stimulated by high glucose in rat mesangial cells, MCP  1 and ICAM  1 secretion. [Keywords:] diabetic nephropathy; Mesangial cell; monocyte chemoattractant protein  1; cell adhesion molecule  1; table epigallocatechin gallate ABSTRACT: Objective To explore the effects of epigallocatechin  3  gallate (EGCG) on proliferation of mesangial cells and the contents of Monocyte chemoattractant protein  1 (MCP  1) and intercellular adhesion molecule  1 (ICAM  1) in high glucose cultured rat mesangial cells. Methods Rat mesangial cells were divided into control group (low gluc