Erk1 - 2 signaling pathway in EGCG inhibited the proliferation of MGC-803 cells the role of.docVIP

 PAGE \* MERGEFORMAT 7 Erk1 / 2 signaling pathway in EGCG inhibited the proliferation of MGC-803 cells the role of [Abstract] Objective To study the form of green tea extract epigallocatechin gallate (epigallocatechin-3-gallate, EGCG) on human gastric cancer MGC-803 cell line proliferation and its molecular mechanism. Methods MTT determination of EGCG inhibited cell proliferation of gastric cancer; the use of immunocytochemistry and Western blot techniques were used to detect c-Myc and phosphorylated Erk1 / 2 protein expression changes. The results of the MGC-803 cells, EGCG inhibited the proliferation, showing dose-dependent. EGCG treatment immunocytochemical detection of c-Myc protein expression down, Western blot detection of phosphorylated Erk1 / 2 protein expression down. Conclusion EGCG inhibited the proliferation of MGC-803 cells in a dose-dependent inhibitory effect of tumor cell proliferation and inhibit Erk1 / 2 phosphorylation and down-regulate c-Myc protein in the. [Keywords:] EGCG; gastric cancer; c-Myc; Erk1 / 2 [Abstract] Objective To probe the effects of green tea extract epigallocatechin-3-gallate (EGCG) on proliferation of human gastric cancer cell line MGC-803 and its molecular mechanisms. Methods MTT assay was used to test the inhibitory effects of EGCG on proliferation in MGC-803 cells. Expression of c-Myc in MGC-803 cells was detected by immunocytochemistry.The expression of phosphorated Erk1 / 2 protein in MGC-803 cells was examined using Western blot analysis.Results MTT assay has shown that EGCG significantly inhibited proliferation of MGC-803 cells in a concentration dependent manner.Immunocytochemistry detected the expression down regulation of c-Myc protein analysis treated with EGCG for 48 hours in MGC-803 cells.Western blot analysis confirmed the expression of phosphorylated Erk1 / 2 protein significantly down regulated.Conclusion EGCG inhibits proliferation of MGC-803 cells in a concentration dependent manner and its mech