Erlotinib on lung cell apoptosis.docVIP

 PAGE \* MERGEFORMAT 17 Erlotinib on lung cell apoptosis Of: Bai Xiaoyan Mu Xiaoyan Jiang Shujuan WANG Ya-li Liu Qingliang [Abstract] Objective erlotinib in human lung adenocarcinoma cell line A549 cell apoptosis as well as epidermal growth factor receptor (EGFR) protein expression. Methods MTT assay different concentrations of erlotinib on A549 cell proliferation; by phase contrast microscopy, flow cytometry, TUNEL method and DAPI fluorescence staining cells; by immunofluorescence analysis of erlotinib on the EGFR protein expression. Results erlotinib A549 growth inhibition was concentration-dependent time; erlotinib treatment after A549 inverted phase contrast microscope, DAPI and TUNEL apoptotic cells were seen significantly (P lt;0.05); drugs to cells treated with obvious G1 arrest ( P lt;0.05); TUNEL detected 200 mol / L erlotinib cell apoptosis rate was (42.13 + -1.4)%, significantly higher (0.82 + -0.03)% (P lt;0.05); immunofluorescence Law Table Minge Luo imatinib significantly decreased expression of EGFR (P lt;0.05). Conclusion erlotinib and the mechanisms of anti-A549 cell-mediated apoptosis, arrest cell cycle arrest in G0/G1 phase and the EGFR signaling pathway. [Keywords:] erlotinib; EGFR; A549 cells; apoptosis [Abstract] Objective To explore the effects of Erlotinib on apoptosis andexpression of EGFR in human pulmonary adenocarcinoma cell line A549 cells. Methods MTT assay was used to determine A549 cells proliferation. Inverted microscope, flow cytometry, DAPI and TUNEL were used to detect apoptosis. The expression of EGFR was determined by immunofluorescence. Results Erlotinib inhibited the growth of A549 cells in a dose dependent and time dependent.The cells treated with Erlotinib showed a typical apoptotic morphology (P lt;0.05) and G1 phase arrestting (P lt;0.05). The apoptosis rate of 200 mol / L Erlotinib group ((42.13 + -1.4)%) was significantly higher than that of control group ((0.82 + -0.03)%) (P lt;0.05). Immunofluorescence