Erigeron nicotine receptors on nerve cells Expression and its neuroprotective effect of.docVIP

 PAGE \* MERGEFORMAT 9 Erigeron nicotine receptors on nerve cells Expression and its neuroprotective effect of Authors: Xiao-Lan Qi, Huang Yong, WANG Yong-lin, Wang Aimin, official Zhizhong [Abstract] Objective To study the traditional Chinese medicine Erigeron right neuroblastoma (SH-SY5Y) cells, neural nicotinic receptor expression and its fight against β -amyloid (Aβ ) neurotoxicity mechanism of action. Methods colorimetric MTT reduction rate of cells was determined by screening the concentration of drug safe range, select a certain concentration of ethanol extract of Erigeron ethyl acetate part of dealing with SH-SY5Y cells, and then join the Aβ 1-42 treatment, measured the rate of MTT reduction, protein blotting (Western blotting) were measured neural nicotinic receptor α 3, α 7 protein expression changes; Ellman colorimetric method using an improved cell culture medium in the acetylcholinesterase (Acetylcholinesterase, AChE) and butyrylcholinesterase (Butyrylcholinesterase , BuChE) activity. The results of ethanol extract of Erigeron parts of ethyl acetate can enhance cell α 3 and α 7 nicotinic receptor subunit protein expression levels; reduce cell acetylcholinesterase activity; and to fight against Aβ induced MTT reduction rate. Conclusion Erigeron ethanol, ethyl acetate extract of nicotine receptor sites to increase the level of subunit protein expression, reducing cell acetylcholinesterase activity, and can fight against the neurotoxicity of Aβ role in the treatment of Alzheimer’s disease may play an to a certain extent. [Keywords:] Erigeron; SH-SY5Y cells; nicotine receptor; β -amyloid protein; cholinesterase; Alzheimer’s disease Abstract: ObjectiveTo investigate the effects of Herba Erigeromtis on the expression of nicotinic acetylcholine receptor (nAChR) and the anti-neurotoxicity mechanism of the drug by using neuroblastoma SH-SY5Y cells treated with beta-amyloid peptide.MethodsThe SH-SY5Y cells were treated by Herba Erigeromitis, and then e