Erythropoietin on A25 ~ 35 cells induced by nerve injury in vitro.docVIP

 PAGE \* MERGEFORMAT 14 Erythropoietin on A25 ~ 35 cells induced by nerve injury in vitro [Abstract] Objective To investigate erythropoietin (EPO on the -amyloid peptide (A induced neuronal toxicity in rats. Methods PC12 cells in logarithmic growth phase were divided into 3 groups: control group, model group, EPO pretreatment group. After 24 h cell culture, EPO pretreatment by adding a final concentration of 25 U / ml of EPO pretreatment 8 h, then with the model group while the final concentration of 10 mol / L A25 ~ 35, cells were collected and cultured for 48 h detection index. using MTT colorimetric analysis of cell viability, propidium iodide (PI apoptosis by flow cytometry, immunohistochemical detection of cytochrome C (CytC and Bcl 2 expression. The results showed EPO treatment MTT cell viability significantly higher than the model group (P lt;0.05), and the highest survival rate in the dose, the apoptosis rate was significantly lower than the model (P lt;0.05), immunohistochemistry showed that: EPO treatment group compared with Bcl 2 positive cells deal with A25 ~ 35 significantly increased (P lt;0.05), while EPO treatment group compared with the model CytC positive staining cells was significantly reduced (P lt;0.05). Conclusion EPO can increase cell survival and reduce apoptosis, induced neural cell toxicity of A play a protective role The mechanism, mainly through increased Bcl 2 expression, thereby inhibiting the CytC release, prevent apoptosis, promote PC12 cell survival. [Keywords:] erythropoietin, A25 ~ 35, PC12 cells, apoptosis Alzheimer’s disease (AD is the older of the most common neurodegenerative disease, clinical manifestations of dementia, cognitive impairment and behavioral abnormalities. Erythropoietin (EPO by the fetal liver and adult kidney secretion of a low molecular weight glycoproteins, regulating the formation of red blood cells. However, the function of EPO are not limited to, EPO and its receptor in the central nervous s