Esophageal cancer gene expression profiles and their correlation with cell differentiation study.docVIP

 PAGE \* MERGEFORMAT 7 Esophageal cancer gene expression profiles and their correlation with cell differentiation study Authors: Pei, Ling Zhiqiang, YANG Hongyan, Huang You-tien, Jimin, DONG Zi-ming [Keywords:] Esophageal cancer Study on gene expression profiles and their relation to cell differentiation in human esophageal squamous cell carcinoma [Abstract] AIM: To investigate the gene expression profiles and to screen the genes related to cell differentiation in primary esophageal cancer, which is helpful to search and locate cell differentiationassociated genes and deepen the understanding of the molecular mechanism of its tumourgenesis. METHODS: cDNA microarray was used to detect the mRNA from both the primary carcinoma and the corresponding esophageal epithelium in 15 cases of human esophageal squamous cell carcinoma (ESCC). The results were analyzed by bioinformatics. RESULTS: Among the 886 target genes, 110 (12.42%) genes were differentially expressed commonly at least twice in all 15 samples. There was a correlation between 16 (1.81%) genes and cell differentiation, of which 9 (1.02%) were upregulated and 7 (0.79%) downregulated. CONCLUSION: Many ESCCassociated genes were screened by highthroughput gene chip method; validating their cellular functions will help to identify the key genes or pathway responsible for ESCC, which might be used as diagnostic markers and therapeutic targets for ESCC. [Keywords] esophageal neoplasms; cDNA microarray; gene expression profile; cell differentiation [Abstract] Objective: To study the gene expression profiles of primary esophageal cancer, screening with tumor cell differentiation-related genes, to find or locate esophageal cancer differentiation-related genes, to deepen their understanding of molecular mechanisms of cancer. Methods: Gene chip technology of 15 cases of ESCC cancer tissues and normal tissues of the mRNA detection, and use bioinformatics to analyze the results of the test. Results: In 886 entries