Experimental intracerebral hemorrhage in rat brain tissue Toll-like receptor 4 expression and significance of.docVIP

 PAGE \* MERGEFORMAT 12 Experimental intracerebral hemorrhage in rat brain tissue Toll-like receptor 4 expression and significance of Author: Li Li Tao, Zhang Jian, Yin Jing, Yang Yi [Abstract] Objective: To investigate the TLR4 around hematoma in rats with cerebral hemorrhage in the expression of brain tissue and their role. Methods: Autologous blood injection method produced tail rat model of cerebral hemorrhage, the 50 SD rats were randomly divided into sham operation group, model group, after modeling at different time points (after 6,24,48,72 h and 7 d) were randomly divided into five sub-groups of five. each group at different time points for subjects using reverse transcription-polymerase chain reaction (RT  PCR) to detect TLR4 mRNA in rat brain tissue relative expression. Results: The experimental group at different time points, both TLR4 mRNA expression of sham-operated group relative expression of TLR4 mRNA at all time points there was no significant differences (Pamp;gt; 0.05); model group and sham-operated group compared with the relative expression of TLR4 mRNA was significantly higher (P amp;lt;0.05), and peaked at 48 h and continued until 72 h. model group, the same point in time the relative expression of TLR4 mRNA compared with the sham-operated group were significantly increased, the difference was statistically significant (P amp;lt;0.01). Conclusion: The rat model of cerebral hemorrhage increased expression of TLR4, suggesting that TLR4 in the inflammatory injury of cerebral hemorrhage may have a role. [Keywords:] cerebral hemorrhage; rat; Toll-like receptor [Abstract] AIM: To explore the expression of Toll  like receptor 4 (TLR4) in brain tissue surrounding hematoma of cerebral hemorrhage rats and its role in brain inflammatory injury. METHODS: The cerebral hemorrhage models of rats were made by injecting autologous arterial blood collected from caudal artery. Fifty male SD rats were randomly divided into 2 groups: sham  operati