Extracellular signal-regulated kinase in the expression of focal cerebral ischemia in.docVIP

 PAGE \* MERGEFORMAT 9 Extracellular signal-regulated kinase in the expression of focal cerebral ischemia in [Keywords:] Cerebral ischemia; reperfusion; mitogen-activated protein kinase; apoptosis; infarction, middle cerebral artery; rat Expression of extracellular signalregulated kinase in focal cerebral ischemia [Abstract] AIM: To study the role of extracellular signalregulated kinase (ERK) during focal cerebral ischemiareperfusion in hippocampal neurons of rats. METHODS: Sixty male adult Wistar rats were randomly divided into 2 groups (n = 30 per group): the sham operation group and the ischemiareperfusion group (I / R group). The rats of I / R group were subjected to 2 h of right middle cerebral artery occlusion (MCAO) with introducing a nylon suture to the right internal carotid artery and then 3, 6, 24, 48 and 72 h of reperfusion, respectively (as 5 subgroups, n = 6 in each subgroup). The histopathologic changes were observed by HE staining. The apoptotic neurons were detected in CA1 area of hippocampus by TUNEL method. ERK phosphorylation was detected by immunohistochemistry. RESULTS: In I / R group as compared with shamoperation group, after MCAO, the survival neurons in CA1 areas decreased; a lot of apoptotic cells were observed and peaked at 48 h; ERK activity increased 3 h after MCAO and peaked at 6 h and returned to the normal level at 72 h. CONCLUSION: Cerebral ischemiareperfusion may result in the upregulation of ERK activity, and participate in the process of neuron apoptosis. [Keywords] brain ischemia; reperfusion; mitogen activated protein kinases; apoptosis; infarction, middle cerebral artery; rats [Abstract] Objective: To study the extracellular signal-regulated kinase (ERK) on focal cerebral ischemia and reperfusion in the expression of hippocampal neurons. Methods: The production line of intraluminal middle cerebral artery occlusion (MCAO) model. Rats were randomly were divided into sham operation group (sham group), cerebral ischemia-