FasL antisense oligonucleotide reversed liver cancer cell immune escape of experimental study.docVIP

 PAGE \* MERGEFORMAT 2 FasL antisense oligonucleotide reversed liver cancer cell immune escape of experimental study Author: Zhang Jiao Liu Qian Wang Jie MAO Hai-ting [Abstract] Objective: To study FasL antisense oligonucleotide on liver cancer cell Fas / FasL way to reverse the role of immune escape. Methods: HCC cell lines by flow cytometry HepG2.2.15 cell surface Fas, FasL expression and Fas expression in Jurkat cell surface; by HepG2.2.15 cells and Jurkat cells co-culture method to study FasL-induced apoptosis of T cells function; by liposome-mediated transduction of FasL antisense oligonucleotide portal triad cancer cells, and by detecting the cell surface FasL expression, RTPCR and cell co-culture method of FasL antisense oligonucleotide transduction of liver reversal of the role of cellular immune suppression. Results: ① HepG2.2.15 cells with low expression of Fas, but expression of function of FasL, with high expression of Fas in Jurkat cells, which can induce apoptosis in co-culture; ② hepatoma cells into the FasL antisense oligonucleotide, FasLmRNA lower ; cell surface FasL expression in decline; and co-cultured Jurkat cells, Jurkat cell apoptosis decreased. Conclusion: The expression of FasL can inhibit liver cancer cells, T cells, immune function, liver cancer cells, immune escape is an important mechanism; FasL transduction of antisense oligonucleotides could reverse the immune inhibition of hepatocellular carcinoma cells. [Keywords:] liver cancer