Focal cerebral ischemic preconditioning on the expression of tumor necrosis factor-experimental study.docVIP

 PAGE \* MERGEFORMAT 7 Focal cerebral ischemic preconditioning on the expression of tumor necrosis factor-experimental study Author: John Wu Shu hard Xiao-Li Liu Jinyu Jiao Shou-feng Ni Naluozuming [Abstract] Objective To observe the focal ischemic preconditioning on rat tumor necrosis factor (TNF-a) the expression of the impact of TNF-a and ischemic tolerance, including the relationship and the role of endogenous protective mechanism. Methods suture method to establish focal cerebral ischemic tolerance in animal models. Selected 30 SD rats, 30 rats were randomly divided into experimental group: ischemic preconditioning (IP MCAO); sham-operated group (SS MCAO): sham-operation instead of IP, I with the experimental group; the control group (SS SS), n = 10. Evaluation indicators include neurological deficit score, and pathological changes under light microscope and immunohistochemical staining and image analysis and comparison of each group changes in the expression of TNF-a. IP can significantly improve the results of focal 3d nerve function in rats after the score, reducing histological damage, reduced TNF-a expression, IOD values of the experimental group (8109.53 ± 571.21) compared sham-operated group (10704.72 ± 584.01) The difference was statistically significant (F = 233.59, P amp;lt;0.05). Conclusion of ischemic preconditioning on focal cerebral infarction followed by a clear protective effect, can induce ischemic tolerance in the production, its possible mechanism is through down-regulating the expression of tumor necrosis factor. [Keywords:] Ischemic preconditioning ischemic tolerance of middle cerebral artery embolization for tumor necrosis factor Abstract Objective To explore the effects of focal cerebral ischemic preconditioning on the expression of tumor necrosis factor-alpha and the relationship of tumor necrosis factor-alpha and ischemic tolerance. Methods The animal model of focal cerebral ischemic tolerance was used. 30 SD rats were divide