Green fluorescent protein marker of Pseudomonas aeruginosa biofilm formation and structure of the dynamic observation of quantitative analysis of.docVIP

 PAGE \* MERGEFORMAT 19 Green fluorescent protein marker of Pseudomonas aeruginosa biofilm formation and structure of the dynamic observation of quantitative analysis of Author: Jia-Lin LIU Guan-Yu Chen Bowman letter Bianca Yan Fang Yang [Abstract] Objective To study the bacteria Pseudomonas aeruginosa biofilm (biofilm, BF) the process of formation and development of spatial three-dimensional structural changes, as well as BF and biological behavior of the process of forming the linkages between. The cells were 6h and 1,3, and 6d to establish four time groups such as Pseudomonas aeruginosa PAO1 strain BF model, through the green fluorescent protein (green fluorescence protein, GFP) tag, combined with laser scanning confocal microscope (confocal laser scanning microscopy, CLSM ) intake of BF various stages of formation and development of different levels of picture pile, after the image structural analysis software (image structure analyer, ISA) analysis was PAO1 strain BF-related spatial structure parameters of quantitative data. Results ① CLSM with GFP tag and realize the dynamics of the PAO1 strain BF formation of the observation; ② ISA software, quantitative analysis shows that, with the BF development, increasing the thickness of the former 3d to increase the degree (19.6μ m) significantly larger than that after the 3d (6.1μ m ), regional porosity (areal porosity, AP), average diffusion distance (average diffusion distance, ADD) and structure of the entropy (textural entropy, TE) in the BF formation of 6h and 6d, respectively: 0.98 ± 0.01 and 0.92 ± 0.02, showing a downward trend; 1.00 ± 0.009 and 1.06 ± 0.027, although not the magnitude of changes, but has also shown a gradual upward trend; 0.7 ± 0.08 and 4.3 ± 0.09, showing a rising trend. Conclusion ISA programs can be characterized PAO1 strain BF spatial structure characteristics and the structure of bacterial BF quantitative analysis will also help to explore the formation and biological beha