H5N1 avian influenza vaccine for human use Effect of immunized animals.docVIP

 PAGE \* MERGEFORMAT 9 H5N1 avian influenza vaccine for human use Effect of immunized animals Author: Zhu Zhiyong, Ding Hang, Zhu Han-ping, LI Yan Jin, Chen Xue-Kui, Shen brothers, Zhang Tao, Pei-jiang, YAO Ping-ping, Xu, WENG Jing-Qing, Gong Yue, Guo Zhijun, Su Bo, Sun Shubin, Joseph LEUNG Keywords: bird flu vaccine; immune animals; neutralizing antibody; the effectiveness of test Abstract: Objective will be used kinds of R1203 strain of bird flu drug production process according to three kinds of vaccines produced for animal inoculation was observed vaccine effect. Methods with different doses of three kinds of processes produce bird flu vaccine, were immunized rats and rabbits, muscle inoculated 2-pin (separated 14d), the first needle 14 and 28d after the venous blood to measure a needle and two needle inoculation, the animals serum hemagglutination inhibition antibody and the production of neutralizing antibodies. The results of the antibody response in rats than in rabbits sensitive; rats and rabbits after vaccination a needle 14d, the blood suppression can produce antibodies titer up to 1:40, but the neutralizing antibody titers were low, almost undetectable; inoculation two needles, the blood and in suppressing antibodies, or antibody responses were significantly higher than the first one needle; cracking -2 seedlings inoculated rats and rabbits after the antibody response is generally higher than the other two kinds of vaccines, and the rabbit volume - response effect apparent. Conclusion cracking -2 better than the other two kinds of vaccines; neutralizing antibody response to the quality of vaccines correctly; H5N1 avian influenza vaccine can be used to test the effectiveness of the determination of the immune rabbit antibody and pre-clinical study of animal testing, using 15μg haemagglutinin (HA) vaccine in rabbits two needles, serum neutralizing antibody titers up to 1:40 or more. Keywords:: Avian influenza vaccines; imm