hNaDC1 gene 5 flanking region of transcriptional regulatory sequences and identification of series of vectors.docVIP

 PAGE \* MERGEFORMAT 11 hNaDC1 gene 5 ‘flanking region of transcriptional regulatory sequences and identification of series of vectors Authors: Zhang Jiankai, Yang Ju-rong, LI Xue-Peng, HE Ya-ni [Abstract] Objective To construct hNaDC1 gene 5 ‘flanking region of transcription regulating sequence series of firefly luciferase reporter gene expression vector. Methods PCR amplification hNaDC1 gene 5 ‘flanking transcriptional regulatory region fragments of different lengths: hNaDC1A (-2 232 / 136,2 368 bp), hNaDC1B (-1 640 / 136,1 776 bp), hNaDC1C (-1 084 / 136 , 1 221 bp), hNaDC1D (-253 / 136,389 bp), hNaDC1E (-2 232/-12, 2 244 bp), with pGL3  Basic for vector hNaDC1 gene 5 ‘flanking sequence of series of deletion plasmids. Recombinant enzyme by specific restriction enzyme digestion, and send sample sequencing. The results successfully constructed hNaDC1 gene 5 ‘flanking region transcriptional regulatory elements firefly luciferase reporter gene expression vector 5: pGL3  hNaDC1A ~ E. Conclusions for further research NaDC1 gene 5 ‘flanking region of the distribution of transcriptional regulatory elements and transcription regulatory elements and transcription factor interactions provide the basic experimental conditions. [Keywords:] hNaDC1 gene regulation of gene expression vector Abstract: Objective To construct the firefly luciferase report gene vectors for 5 ‘flanking regulated elements of hNaDC1 gene. MethodsThe DNA fragments hNaDC1A ~ E (-2 232 / 136) in 5’flanking region of hNaDC1 gene were amplified from the nephridial tissue by using PCR. The PCR products were directionally subcloned into pGL3  Basic vector. The recombined clones were identified by agarose gel electrophoresis after restriction endonuclease digesting and DNA sequencing. Results 5 expression vectors (pGL3  NaDC1A ~ E) for 5 ‘flanking regulated elements of hNaDC1 gene had been constructed. ConclusionThese expression vectors offer the basic experimental conditions for studying the