High-performance liquid chromatography - evaporative light scattering particles in the Qing Tong Chuan Astragaloside content.docVIP

 PAGE \* MERGEFORMAT 8 High-performance liquid chromatography - evaporative light scattering particles in the Qing Tong Chuan Astragaloside content Author: Zhu Ling-ying, Duan Jin-ao, Qian Dawei, JU Jian-Ming [Keywords:] Tong Chuan-ching particle;,,,, astragaloside;,,,, high performance liquid chromatography Abstract: Objective To establish the child breathe a clear determination of particle concentration in high-performance liquid chromatography - evaporative light scattering (HPLC-ELSD) method. Methods HANBANG ODS C18 (4.6 mm * 250 mm, 5 μ m) column; column temperature: 30 ℃ ; mobile phase of acetonitrile - water (36:64), flow rate 1.0 ml / min; evaporative light scattering detector (drift tube temperature: 110 ℃ , gas flow rate: 2.3 L / min) detection. Results astragaloside volume of 2.036 ~ 20.460μ g showed good linear relationship within the framework of, r = 0.999 1, the sample average recovery was 97.8%, RSD 1.97%. Conclusion HPLC-ELSD determination of child asthma Qing Granules Astragaloside accurate and reliable, rapid, sensitive and reproducible. Keywords:: Children’s asthma Qing particle; Astragaloside; high-performance liquid chromatography Determination of Astragaloside IV in Tongchuanqing Granules by HPLC-ELSD Abstract: ObjectiveTo established an HPLC-ELSD method for the determination of astragalosid IV in Tongchuanqin Granules.MethodsA Hanbon C18 (4.6 mm * 250 mm, 5 μ m) column was used and a mixture of acetonitrile-water (36:64) was used as the mobile phase at a flow rate 1.0 ml / min.The column temperature was 110 ℃ and the flowrate was 2.3 L / min.ResultsThe linear ranges of determination of astragalosid IV were 2.036 ~ 20.460 μ g, and r was 0.999 1.The average recovery was 97.8% and RSD was 1.97%. ConclusionThe method is sensitive, rapid and the results are accurate with good reproducibility. Keywords:: Tongchuanqin Granules; Astragaloside IV; HPLC-ELSD Tong Chuan-ching of Chinese medicine granules preparation for the new R amp;amp