High-performance liquid chromatography - mass spectrometry determination of carbendazim residues in fruits and vegetables in the amount of Method.docVIP

 PAGE \* MERGEFORMAT 7 High-performance liquid chromatography - mass spectrometry determination of carbendazim residues in fruits and vegetables in the amount of Method [Keywords:] high-performance liquid chromatography-mass spectrometry of carbendazim residues in fruits and vegetables Carbendazim (Carbendazim) also known as benzo imidazole 44, the chemical name is 2 - (methoxy-carbamoyl) benzo imidazole, is a broad-spectrum, efficient absorption of fungicides within pesticides, to Sclerotinia sclerotiorum , anthracnose, fusarium wilt and others have control effect has been widely used in fruit and vegetable production. Because of its chemical stability, can be lasting residue in fruits and vegetables, resulting in accumulation of carbendazim residue could affect human health through the food chain. Studies have shown that carbendazim can cause liver disease, can lead to chromosome aberrations and other diseases [1]. Determination of Carbendazim in the current analysis methods are mainly liquid chromatography [2,3], spectrophotometric [4]. Commonly used high performance liquid chromatography UV detector, because of their poor selectivity, sensitivity is limited, and different samples have different chromatographic conditions, the detection of significant interference, methods, versatility and durability is not strong. To this end, the present study, high selectivity and high sensitivity of the MS detector to common fruits and vegetables as the research object, the establishment of carbendazim residue detection liquid chromatography - mass spectrometry method. Experimental part Main instruments and reagents: 1100-type high-performance liquid chromatography - mass spectrometer (Agilent Technology Co., Ltd.), equipped with machine-line degasser, quaternary low pressure pump, autosampler, column temperature box, diode array detector, EFI Fog quadrupole mass spectrometry detector, instrument control and data acquisition and analysis using the ChemStation worksta