High-performance liquid chromatography Baotai Tablets paeoniflorin.docVIP

High-performance liquid chromatography Baotai Tablets paeoniflorin.doc

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High-performance liquid chromatography Baotai Tablets paeoniflorin

 PAGE \* MERGEFORMAT 9 High-performance liquid chromatography Baotai Tablets paeoniflorin Author: Ding Yufen Cheng-Juan Li Guiming [Abstract] Objective: A high performance liquid chromatography Baotai Tablets paeoniflorin. Methods: KromasilC18 (4.6mm * 250mm, 5μ m) column; mobile phase of acetonitrile - water (ten ninety); detection wavelength 230nm; column temperature 40 ℃ ; a flow rate of 1.0ml * min-1. Results: The sample volume of paeoniflorin 0.1572μ g ~ 0.7860 within the linear regression equation Y = 98073.5000X-17548.2000 (r = 0.9998). The average recovery was 100.04%, RSD of 0.7%. Conclusion: This method is simple, rapid, sensitive, accurate and can amend the preparation for future quality standards provide a reference. [Keywords:] Baotai Tablets Determination of paeoniflorin in HPLC method Determination of the Contene of Baotai LingTableta by HPLC [Abstract Objective] To establish a HPLC method for determination of the content for Paeoniflorin of Baotai Ling Tableta. Method: A Kromasil C18 column (250mm * 4.6mm, 5μ m) was uesd.and temperature was set at 40 ℃ . The mobile phase consisted of Acetonitrile-Water (10:90), with flow rate of 1.0ml * min-1.The UV detection wavelength was set at230nm.Results: The calibration curves of the Paeoniflorin were linear in the range of 0.1572μ g ~ 0.7860μ g (r = 0.9998). Conclusion: This method apears to be simple, rapid, sensitive and accurate. [Keywords:] Baotai Ling Tableta; Paeoniflorin; HPLC; content determination. Baotai Tablets is a kind of kidney, solid red, tocolysis of pure Chinese medicine used for threatened abortion, habitual abortion and infertility caused by abortion. For long-term infertility or miscarriage pregnancy after infertility who can promote the role of the body simultaneously to enhance immunity and promote the effectiveness of fetal growth, non-toxic side effect, should long-term use. Closed its quality standards are set out in ‘The Ministry of Health awarded the standard of Chin

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