High-performance liquid chromatography - evaporative light scattering detection Determination of chlorogenic acid in Lonicera japonica and luteolin glycosides content.docVIP

 PAGE \* MERGEFORMAT 12 High-performance liquid chromatography - evaporative light scattering detection Determination of chlorogenic acid in Lonicera japonica and luteolin glycosides content Author: Kurban, Jiangsu Wen-Qin Zhang Peng-wei [Abstract] Objective To establish a high-performance liquid chromatography - evaporative light scattering detection (HPLC-ELSD) Honeysuckle Determination of chlorogenic acid and luteolin glycosides method. Methods Chromasil C18 column (4.6 mm * 250 mm, 5.0μ m) for the column, with acetonitrile (A) -0.5% acetic acid (B) as mobile phase gradient elution in order to evaporative light scattering detector and chlorogenic acid and luteolin glycosides. Gradient procedure: 0 min (A: 10%, B: 90%) -16 min (A: 25%, B: 75%) -22 min (A: 50%, B: 50%); flow rate of 1.0 ml * min-1; detector drift tube temperature of 110 ℃ , carrier gas flow rate 3.0 ml * min-1. The results of chlorogenic acid and luteolin glycosides regression equations were: lgA = 1.34lgm 4.360, r = 0.999 5; lgA = 1.653lgm 5.327, r = 0.999 6. Linear in the range of 5.0 ~ 45 μ g, and 0.4 ~ 3.6 μ g; average recoveries were 99.94% (RSD = 1.43%, n = 5) and 100.85% (RSD = 1.78%, n = 5). Conclusion The method is simple, accurate, specific and strong, with good separation efficiency and reproducibility, which can effectively reduce the total analysis time. Honeysuckle for a more complete control of the quality control of medicinal herbs provide a simple and reliable method. [Keywords:] Honeysuckle chlorogenic acid, luteolin glycosides by high performance liquid chromatography - Evaporative Light Scattering Detector HPLC Abstract: ObjectiveTo develop a method for determination of chlorogenic acid (ChA) and galuteolin (Gal) in Flos Lonicerae Japonicae.MethodsAn HPLC equipped with ELSD method was applied to quantify chlorogenic acid and galuteolin. The analytical column was Chromasil C18 (4.6 mm * 250 mm, 5.0μ m) with the temperature of 30 ℃ . The chromatogram for the ChA and Gal