HPLC determination of biological samples of cefotaxime and sulbactam concentrations.docVIP

 PAGE \* MERGEFORMAT 16 HPLC determination of biological samples of cefotaxime and sulbactam concentrations Authors: Yi Gu, Xiao-Juan, Wang Rong, Bai Juanjuan, high-Su-li [Keywords:] cefotaxime; Sulbactam; chromatography, high pressure liquid; plasma; urine [Abstract] AIM: To establish a determination method of concentrations in human biologic sample following intravenous administration of cefotaxime and sulbactam. METHODS: A RPHPLC method was developed and validated to determine cefotaxime and sulbactam in plasma and urine. The mobile phase of cefotaxime and sulbactam consisted of 1 g / L triethylamine (regulated with phosphate acid to pH = 6.2) and methanol in a 68:32 and 84:16 (v / v) respectively and pumped at a flow rate of 1 mL / min. The plasma samples were deproteinated by addition of methanol, the urine samples were diluted with super pure water, then 20 μ L of supernatant was injected onto a C18 column and monitored with UV detector at 254 nm and 220 nm respectively. RESULTS: The assay of cefotaxime in human plasma and urine were linear over the range of 5.3-530.0 mg / L and 5.45-545.00 mg / L respectively, with the detection limit of 1 mg / L and 0.25 mg / L respectively. The assay of sulbactam in human plasma and urine were linear over the range of 1.04-208.00 mg / L, with the detection limit of 0.50 mg / L and 0.25 mg / L respectively. The average recovery of the method was (89-110)%, and the RSD within and between days were all less than 5%. The cefotaxime and sulbactam sample of plasma and urine stored in a -20 ℃ refrigerator became stable within 3 d. CONCLUSION: The assay method is rapid, accurate and usable for the determination of cefotaxime and sulbactam concentrations in plasma and urine. [Keywords] cefotaxime; sulbactam; chromatography, high pressure liquid; plasma; urine [Abstract] Objective: In order to intravenous injection of cefotaxime sodium and sulbactam sodium in plasma and urine after the drug concentration analysis detecti