HPLC determination of baicalin in Shuanghuanglian oral content of.docVIP

 PAGE \* MERGEFORMAT 7 HPLC determination of baicalin in Shuanghuanglian oral content of [Abstract] Objective To HPLC Determination of baicalin in Shuanghuanglian oral content. Methods 18 alkyl bonded silica gel as the stationary phase; methanol: water: glacial acetic acid (60:50:1) as mobile phase; flow rate of 1.0ml/min; detection wavelength 274nm. The results of baicalin in 25 ~ 220μ g showed good linearity within the scope (r = 0.9995); average recovery was 100.59%, RSD of 1.02% (n = 9). Conclusion The method is rapid, accurate separation of impurities intact, reproducible, and can be used for the preparation in the determination of baicalin. [Keywords:] high-performance liquid chromatography; baicalin; Shuanghuanglian oral solution Shuanghuanglian oral prescriptions containing honeysuckle, Scutellaria, and forsythia, with Xin Liang Xie table, Qingrejiedu of efficacy for the treatment of exogenous wind-heat caused by fever, cough, sore throat and the symptoms. Chinese Pharmacopoeia [1] using HPLC Determination of baicalin in Shuanghuanglian oral content as indicator of the quality control of preparation, the results of the sample baicalin peak and impurity peaks can not be completely separated (Figure 1). This determination by changing the proportion of mobile phase, resulting separation is good, fast, accurate, reproducible, and can be used in the preparation of quality control. An instrument and reagent 1.1 Instruments USA SSI Ⅳ -type liquid pump, M525 UV detector, Anastar chromatography workstation; BP-211D Sartorius electronic balance. 1.2 reagent baicalin reference substance (China Pharmaceutical and Biological Products Institution, batch number 200512); Shuanghuanglian Oral (Kazakhstan White Swan High-Tech Pharmaceutical Group Co., Ltd., batch number 05117-1; Jiangsu Suzhou Changzheng Wu Industrial Co., Ltd. Pharmaceutical Factory, batch number 050,114; China’s Heilongjiang Wandashan pharmaceutical, batch number 050,301); methanol (HPLC pure), g