HPLC determination of berberine hydrochloride in Huangbai Capsule content.docVIP

 PAGE \* MERGEFORMAT 6 HPLC determination of berberine hydrochloride in Huangbai Capsule content Author: Yu Xiaohui, Zhao Lei, Wu Fuxiang, Liu Ben-Liang Abstract: Objective: To establish Huangbai capsule method for the determination of berberine hydrochloride. Methods: The high-performance liquid chromatography (HPLC) determination of berberine hydrochloride. Results: Berberine 0.05 ~ 0.5 μ g in the context of a good linear relationship, r = 0.9998, average recovery was 96.9%, RSD = 1.43% (n = 5). Conclusion: The method is simple, accurate, reliable, and can be used for quality control of Huangbai Capsule. Keywords:: high-performance liquid chromatography; Huangbai Capsule; berberine Phellodendron Capsule is made of a single Huangbai taste traditional Chinese medicine preparation, with heat dampness, Xiehuo In addition to steam, boil and so the effectiveness of detoxification treatment in clinical practice is mainly used for hot and humid Xie Li, jaundice, vaginal discharge, hot shower, etc., to close contained in the ‘The Ministry of Health Drug Standards’ (traditional Chinese medicine set prescription preparations, Volume V). The main drug Huangbai berberine in one of the components of its anti-bacterial 〔 1〕 , its quality standards, only Huangbai qualitative identification, for the effective control of the drug quality, this paper, RP-HPLC determination of the main drug Phellodendron berberine content, this method is simple, accurate and reproducible, the results are less satisfactory. An instrument and reagent SP 8810 high performance liquid chromatography, SP 200 detector, SP 4600 data-processing instrument (USA Spectra-Physics Inc.), berberine (identification of Pharmaceutical and Biological Products), acetonitrile (HPLC pure reagent), re-distilled water, other reagents were analytical pure. Samples of commercially available products (Batch No:20010806. 2 Methods and Results 〔〕 2-4 2.1 Chromatographic conditions C18 reversed-