HPLC determination of bitter bitter Figwort Scrophulariaceae Glycosides IA content.docVIP

 PAGE \* MERGEFORMAT 10 HPLC determination of bitter bitter Figwort Scrophulariaceae Glycosides IA content Author: Chen Yong, Han-Shen Zhen, Liu Jing, Li Yaohua, Ye Le [Abstract] Objective To establish the bitter herbs figwort Picria fel  terrae Lour. Method for the determination. Methods RP-HPLC method, using C18ODS2 column (5μ m, 4.6 * 250mm), acetonitrile -0.3% phosphoric acid (34:66) as mobile phase at a flow rate 1ml/min, detection wavelength 264nm. Results bitter Scrophulariaceae Glycosides IA injection volume of 0.42 ~ 2.10μ g within the framework of a good linear relationship, r = 0.9999, average recovery was 100.4%, RSD = 1.64% (n = 6). Conclusion The method is simple, accurate, specific and can be used as medicine in the bitter bitter Figwort Scrophulariaceae Glycosides IA quality control methods. [Keywords:] hard Scrophulariaceae; bitter Scrophulariaceae Glycosides IA; Determination; high performance liquid chromatography [Abstract] Objective To establish a method for the determination in picria fel-terrae Lour.Methods RP-HPLC.The piefeltarraeninIA was separated on a C18ODS2 column (5μ m, 4.6 * 250mm) and detected at 264nm and 1ml/min, using acetonitrile - 0.3% phosphoric acid (34:66) as the mobile phase. Results PiefeltarraeninIA in picria fel  terrae Lour was baseline separated and determined.The linearity rangs was 0.48μ g ~ 2.10μ g, correlation coefficient was 0.9999.The average recovery was 100.4% ( RSD = 1.64%, n = 6). Conclusion The method is simple, accurate and with strong specificity and can be used for quantify the main constituient piefeltarraeninIA in picria fel  terrae Lour. [Keywords:] picria fel-terrae Lour; piefeltarraeninIA; content determination; high performance liquid chromatography (HPLC) Bitter Scrophulariaceae Picria fel-terrae Lour. For the suffering Scrophularia Scrophulariaceae only a plant, mainly distributed in Guangxi, Guangdong and Yunnan and other places. As a folk medicine in Guangxi has been 200