HPLC determination of caffeic acid spray Qingyan Ning Content.docVIP

 PAGE \* MERGEFORMAT 9 HPLC determination of caffeic acid spray Qingyan Ning Content [Abstract] Objective To determine the Qingyan Ning spray method of caffeic acid. Methods using high performance liquid chromatography method was used according to Litt BDS C18 column (200 mm * 5.0 mm, 5 μ m), methanol  0.013 mol / L phosphate buffer (volume ratio seventy-seven past eleven p.m.) as the mobile phase; flow rate of 1.0 mL / min, detection wavelength was 320 nm. The results in the range of 0.042 5 ~ 0.425 μ g, the peak area and injection volume was a good linear relationship (r = 0.999 9), plus sample average recovery was 99.05%, RSD 2.9%. Conclusion The method is simple, reliable, accurate and satisfactory results. [Keywords:] HPLC; Qingyan Ning spray; caffeic acid; Determination Determination of caffeic acid in Qingyanning spray by HPLC Abstract: Objective To establish an HPLC method for determination of caffeic acid in Qingyanning spray. Methods The sample was analyzed on a BDS C18 column (200 mm * 5.0 mm, 5μ m) using methanol  0.013 mol / L phosphate salt solution (23:77 ) as the mobile phase. The flow rate was 1.0 mL / min and the detection wavelength 320 nm.Results The method exhibited good linearity from 0.042 5 to 0.425 μ g (r = 0.999 9). The average recovery was 99.05% and RSD 2.9% . Conclusion The method is convenient, reliable and accurate for the quality control of the spray. Keywords:: HPLC; Qingyanning spray; caffeic acid Ning is the main by Qingyan spray dandelion, Caulis Lonicerae composed of pure Chinese medicine, with heat detoxification, anti-inflammatory pain and other effects, can be used to treat stomatitis, oral ulcers, pharyngitis embolism, oral inflammation in children with special effects. Caffeic acid as a dandelion, Caulis Lonicerae two antibacterial drugs, one of the active ingredients of anti-virus [1], easily measured. Therefore, this preparation reference to ‘‘2000 edition of Chinese Pharmacopoeia method for the determinatio