HPLC Determination of complex renal Capsules paeonol.docVIP

 PAGE \* MERGEFORMAT 6 HPLC Determination of complex renal Capsules paeonol Author: Ya-li, Chen Yafen, LIU Jing-xia, Wang Guohua [Abstract] Objective: To establish a complex renal capsules paeonol determination method. Methods: Determination of HPLC, column: Kromasil 5μ C18; mobile phase: methanol - water (65:35); detection wavelength: 274nm. Results: The linear range of determination of paeonol 0.03864-0.5796μ g, r = 1.0000, average recovery was 99.6%, RSD = 0.95%. Conclusion: This method is sensitive, rapid, accurate and can be used to measure the re-Shen Ning in paeonol. [Keywords:] complex kidney capsule Paeonol high-performance liquid chromatography assay An instrument and reagent High-performance liquid chromatography: LC  2010 high-performance liquid chromatography (Shimadzu, Japan); paeonol reference substance (for determination of use, batch number: 0708  9704) purchased in China pharmaceutical and biological products; Fu Shen Ning capsule samples are refined in Jilin Province Xin Pharmaceutical Group Co., Ltd. production; the acquisition of flavor ingredients in the medicine company in Jilin Province; mobile phase for chromatography of methanol alcohol, and other reagents of analytical grade. 2 Determination 2.1 Chromatographic conditions [1] Column: A Kromasil 5μ C18; mobile phase: methanol - water (65:35); flow rate: 0.7ml/min; baseline separation of peaks. Detection wavelength: the reference substance Paeonol UV solution for scanning, the maximum absorption of 274nm, and made reference to the Chinese Pharmacopoeia paeonol measured wavelength, so to choose their measured wavelength of 274nm. Theoretical plate number calculated by paeonol peaks should not be less than 3000. 2.2 for the test conditions of extraction and purification of the choice of goods, the sample with methanol ultrasonic ① ; ② the samples were steam distillation. The method ① , ② comparison between the two calculated levels were similar, but the method ① easier, s