HPLC Determination of Compound Rhubarb hemorrhoid suppository emodin content of Chrysophanol.docVIP

 PAGE \* MERGEFORMAT 8 HPLC Determination of Compound Rhubarb hemorrhoid suppository emodin content of Chrysophanol [Abstract] Objective To establish a RP-HPLC Determination of Compound Rhubarb hemorrhoid suppository emodin, chrysophanol content approach. Methods The analytical column was Kromasil C18 (250 mm * 4.6 mm, 5 um), with methanol -1% phosphoric acid (85:15) as mobile phase, flow rate of 1.0 ml * min-1, detection wavelength of 254 nm. The results of emodin, chrysophanol respectively 0.003 944 ~ 0.039 44 μ g, 0.008 128 ~ 0.081 28 μ g good linear relationship between the recoveries were 100.5% and 96.6%, RSD were 0.01% and 1.16% (n = 6). Conclusion The method is simple, high sensitivity, good reproducibility can be used for hemorrhoid suppository emodin compound rhubarb, rhubarb determination of phenol. [Keywords:] compound rhubarb hemorrhoid suppository emodin chrysophanol high-performance liquid chromatography Abstract: ObjectiveTo establish an HPLC method for determination of the contents of emodin and chrysophanol in Dahuangzhichuang suppository. MethodsThe Thermo Kromasil C18 column was used.The mobile phase was CH3OH-H2O 0.1% phosphorie acid solution (85:15), the flow rate was 1.0 ml * min-1 and the detection wavelength was at 254nm.ResultsThe linear ranges of emodin and chrysophanol were at 0.003 944 ~ 0.039 44 μ g (r = 0.999 9), and 0.008 128 ~ 0.081 28 μ g (0.999 9), the average recoveries were 100.5% (RSD = 0.01%, n = 6) and 96.6% (RSD = 1.16%, n = 6), respectively.ConclusionThe method is reliable and accurate. Keywords:: Dahuangzhichuang suppository; Emodin; Chysohanol; RP-HPLC Hemorrhoids suppository compound rhubarb mainly by Shi Di, rhubarb, mirabilite, snail shell (fried), Olive (fried charcoal), borneol and other traditional Chinese medicine, has a heat defecation, bleeding, swelling and pain relief, removal of the convergence of fixed effects. For the period of internal hemorrhoids, mixed hemorrhoids hemorrhoid’s part, mild prolap