HPLC determination of methionine and Vitamin B1 injection of methionine and vitamin B1 content of.docVIP

 PAGE \* MERGEFORMAT 8 HPLC determination of methionine and Vitamin B1 injection of methionine and vitamin B1 content of [Abstract] Objective To determine the methionine and Vitamin B1 injection of methionine and Vitamin B1 content of the method. Way preclude the use of high-performance liquid chromatography, column: ODS  C18 column (4.6 mm * 250 mm, 5 μ m), mobile phase: buffer solution [sodium check heptane 0.25 g, with sodium phosphate solution (taking sodium phosphate 38 g, add water to 1 000 mL allows the dissolved, with phosphoric acid adjusted to pH value of 2.1), dissolved and diluted to 1 000 mL]  acetonitrile (volume ratio 94:6), flow rate 1.0 mL / min, detection wavelength at 21 0nm. Results-L-methionine at 0.60 ~ 1.40 mg / mL concentration range a good linear relationship (r = 0.999 6), vitamin B1 at 0.06 ~ 0.14 mg / mL concentration range a good linear relationship (r = 0.999 8); average recovery are as follows: methionine 100.49% (RSD 0.62%), vitamin B1 99.95% (RSD 0.48%). Conclusion The method is simple, rapid, sensitive, accurate, reproducible, can be used as methionine and Vitamin B1 injection of methionine and method for the determination of vitamin B1. [Keywords:] high-performance liquid chromatography; injection Methionine Vitamin B1; L-methionine; Vitamin B1 Injection of methionine and methionine and Vitamin B1 Vitamin B1 complex preparations. This product is for acute and chronic hepatitis, cirrhosis, especially for fatty liver has a special effect; can be used for intrahepatic cholestasis, as well as alcohol, barbiturates, sulfonamides, when the adjuvant treatment of drug poisoning; can be as a treatment for neuritis and myocarditis supplementary drug. Its agents and freeze-dried powder injection of the current Determination has been reported in the literature [1-3]. Order to effectively control product quality, this direct use of HPLC determination of its main components methionine and vitamin B1 content, method is simple and accu