HPLC determination of rhino Ling detoxification pills glycyrrhizic acid.docVIP

 PAGE \* MERGEFORMAT 10 HPLC determination of rhino Ling detoxification pills glycyrrhizic acid Study: War Qin Yong, Lin Lili, Yao snow, Du Tree Hill [Abstract] Objective To establish a rhino Ling Wan detoxification method for determination of glycyrrhizic acid. Methods HPLC, column: Diamonsil C18 (250 mm × 4.6 mm, 5 μm), mobile phase: methanol - [0.2 mol / L ammonium acetate - acetic acid (33:1)] (64:36), detection wavelength: 250 nm, flow rate: 1.0 mL / min. Results licorice Ammonium range of 0.075 ~ 0.375 μg showed a good linear relationship (r = 0.999 6 ), recovery was 98.25%, RSD = 0.92%. Conclusion The method is rapid, simple, accurate, reproducible, reliable and can be used to control the rhinoceros Ling detoxification pills drug product. [Keywords:] rhino Ling detoxification pills, acid, high performance liquid chromatography, determination Abstract: Objective To establish a method for the determination of Glycyrrhizic acid in Xiling jiedu Pills. Methods Diamonsil C18 (250 mm × 4.6 mm, 5 μm) column in an oven at 30 ℃ was need, with a mobile phase consisting of methanol - [ 0.02 mol / L ammonium acetate - glacial acetic acid (33:1)] (64:36) and a UV detector at 250 nm, the flow rate was 1.0 mL / min. Results The linear range of Glycyrrhizic acid was 0.075 ~ 0.375 μg / mL. The average recovery was 98.25% (RSD = 0.92%). Conclusion The method is simple and accurate, and can be used for the quality control of Xiling Jiedu Pills. Keywords:: Xiling Jiedu Pills, Glycyrrhizic acid, HPLC, content determination Ling detoxification pills rhino is Scrophulariaceae, forsythia, mint, Arctiin (fried), Nepeta ear, Radix, Lonicera japonica, Rehmannia, Ophiopogon, gardenia, pale leaves, bellflower, licorice root, rhinoceros horn powder, buffalo horn powder concentrate , antelope horn powder, borneol, composed of pure Chinese medicine, has published Xinliang, remove the effect of plague toxin, when used for evil caused by exogenous wind evil fever, head