HPLC determination of tranilast eyedrops in the tranilast and its related substances in.docVIP

 PAGE \* MERGEFORMAT 15 HPLC determination of tranilast eyedrops in the tranilast and its related substances in 【Abstract】 Objective: To establish a high-performance liquid chromatography determination of tranilast eyedrops in the tranilast and its related substances in the content. Methods: HANBANG Lichrospher C18 (200mm × 4.6mm, 5μm) for the column with methanol-0.02mol * L-1 potassium dihydrogen phosphate solution (pH value is adjusted with phosphoric acid to 4.2) (60:40) as mobile phase, UV detection wavelength of 333nm, flow rate 1.0ml * min-1. Results: Tranilast in the range of 25 ~ 200mg * Ll has good linear relationship (r = 0.999 9), the average recovery was 98.86%, RSD 1.10%; three batches of samples of the marked percentage content (% ) 99.57,100.17 and 99.87, respectively, the percentage content of related substances, respectively 0.52,0.56 and 0.55. Conclusion: This method is accurate, sensitive, good reproducibility can be used tranilast eye drops and its related substances by HPLC. Keywords: tranilast eye drops related substances by high performance liquid chromatography Tranilast (tranilast, cinnamon anthranilic acid) as the new anti-allergic drugs may stabilize mast cells and macrophages alkaline membrane to prevent cell crack relief particles, thus inhibiting histamine, 5  HT, leukotrienes and SRS  A and other allergic mediators, at present, mainly used in clinical allergic asthma, allergic rhinitis and allergic dermatitis treatment and prevention [1]. The 20th century, the first 70 years by the Japanese scholar Koda et al [2] was successfully developed; internally by the successful development of China Pharmaceutical University, and the only oral agent production and sales, there is no of its eye drops (treatment of allergic conjunctivitis), for Here we develop tranilast eyedrops, for the control of its quality, in reference [3] is established on the basis of the determination of tranilast eye drops and its related subs