HPLC determination of tuberculosis chamaejasme B Tablets by HPLC.docVIP

 PAGE \* MERGEFORMAT 8 HPLC determination of tuberculosis chamaejasme B Tablets by HPLC [Abstract] Objective To determine the tuberculosis Tablets chamaejasme B pigment content. Methods using high performance liquid chromatography column was Agilent HC C18, mobile phase of methanol water (70:30 volume ratio, flow rate 1.0 mL / min The detection wavelength was 291 nm, nm. Results Euphorbia factor B concentration in the 0.606 ~ 7.278 g / mL and the peak area within the linear relationship, r = 0.999 8, average recovery was 100.20%, RSD was 0.29% (n = 6. Conclusion The method is simple, accurate and can be used to determine the TB Tablets chamaejasme B by HPLC. [Keywords:] TB Tablets chamaejasme B prime HPLC Tuberculosis Tablet closing contained in the lt;lt;Health Department drug standardsgt;gt; Volume XIII in the Chinese prescription preparations, from herbs Euphorbia (Euphorbiaceae plant Euphorbia ebracteolata Euphorbia ebracteolata Hayata and Euphorbia Euphorbia Euphorbia fischeriana Steud.’s purified by boiling the root from, attending lymphatic system, tuberculosis, and other lymphatic also effective [1]. The standard TB using ultraviolet spectrophotometry B Tablets of the Euphorbia factor content. This paper established a high-performance liquid chromatography and external standard method, the results show that the method is simple, accurate and reliable, the content of the preparation method. An instrument and reagent SHIMADZU Shimadzu LC 2010A high performance liquid chromatography, CLASS VP chromatography data workstations, SartoriusBP211D electronic analytical balance, JL 180DTH ultrasonic cleaner. TB Tablets (Jiangmen City Health amp; Pharmaceutical Limited, batch number: 070701,071101,080201,080501,080801,081001. Chamaejasme B element standard (based drugs by the Institute of Jiangxi Province in limited liability companies, puritygt; = 99.9%, batch number: 461130601, methanol (Tianjin Four Friends, HPLC grade double distilled water