HT036 is the interaction between P311 fibrosis-related protein gene.docVIP

 PAGE \* MERGEFORMAT 13 HT036 is the interaction between P311 fibrosis-related protein gene Of: Peng Xu, Tanjiang Lin, Yuan Shunzong, feng, Chen en Wei, Luo Gaoxing, Wu Jun [Abstract] Objective To observe the laser confocal microscope unknown protein (HT036) and fibrosis-related protein (P311) and common expression of the intracellular localization. Was constructed with green fluorescent HT036 eukaryotic expression vector, was identified correctly, and pre-construction red fluorescence of the P311 with the eukaryotic expression vector were transfected into human embryonic kidney epithelial cell line (HEK293), while co-transfected with empty vector as control, were observed by confocal microscopy the expression of proteins in cells, the distribution and co-localization. Results HT036 eukaryotic expression vector by polymerase chain reaction (PCR), restriction enzyme digestion and sequencing, and P311 were transfected HEK293 cells, confocal microscopy the expression of green and red fluorescence. HT036 and P311 cells were mainly distributed in the cell pulp, both in HEK293 cells were co location, while the control group were not the phenomenon. Conclusion The yeast two-hybrid interacting proteins out of the P311 HT036, the memory in living cells co-localized in space, between them, further evidence the interaction of P311 for the new entry point fibrotic mechanisms. [Keywords:] wound repair; protein; gene; fibroblasts; scar Abstract: Objective To observe the intracellular expression and the co location of HT036 with P311 in human embryo kidney cell line.Methods The encoding sequence of HT036 was amplified and cloned into the eukaryotic expression plasmid pEGFP N2.The construction was verified by sequencing and then co transfected into HEK293 with pDsRed P311.The expression products were visualized and analyzed by confocal microscope.Results The vector was successfully constructed.The confocal microscope scanning showed that the fluorescence fusion pro