Human angiogenin Eukaryotic expression and subcellular localization of.docVIP

 PAGE \* MERGEFORMAT 10 Human angiogenin Eukaryotic expression and subcellular localization of Authors: ZHANG Hong-bin, WU Jie, Yang Tai-Cheng, Jiang Xian, Huang-Wen Lai, Chuan-Hong Yang, Wen-Ling Zheng [Keywords:] of human angiopoietin;, eukaryotic expression;, positioning;, nucleus [Abstract] Objective: To establish a human angiopoietin (angiogenin, ANG) eukaryotic expression system and Guinea in the cell location. Methods: Recombinant subclone technique fluorescent eukaryotic expression plasmid pEGFP / ANG, and then by the liposome-mediated transfection of human umbilical vein endothelial cells (HUVEC) and cells transfected with pEGFP / ANG expression by fluorescence microscope and were identified by immunohistochemical staining. Using confocal microscopy and immune electron microscopy of the subcellular localization of ANG. Results: Fluorescence microscopy of HUVEC transfected visible to issue strong green fluorescence. Immunohistochemical staining showed that transfection of HUVEC have brown particles. Confocal microscopy and immuno-electron microscopy showed that, ANG concentration in the nuclear area. Conclusion: The Construction of recombinant pEGFP / ANG transfected HUVEC may, after expressing the human ANG protein; the expression of ANG located in the nucleus area. [Keywords:] of human angiopoietin; eukaryotic expression; positioning; nuclear Angiogenin (angiogenin, ANG) is a relative molecular mass (Mr) of 14100, pI 9.5, from 123 amino acids of the protein present in normal plasma and solid tumor tissues belonging to RNase superfamily members [1], its gene location on chromosome 14 in q11 area. ANG has a strong role in promoting angiogenesis. A large number of studies have shown that, ANG and rapid tumor growth and metastasis are closely related to [2, 3], lung cancer, pancreatic cancer and breast cancer in patients with peripheral blood levels of ANG was significantly higher, and the serum levels of ANG with these tumors the development and tra