Human bone marrow mesenchymal stem cell isolation culture differentiation and identification of.docVIP

 PAGE \* MERGEFORMAT 14 Human bone marrow mesenchymal stem cell isolation culture differentiation and identification of Of: Yang Honghui Gao Chuan Po Xiao Yu Li Chuanchang Group to Chen Yan Xu [Abstract] Objective To investigate the human bone marrow mesenchymal stem cells (hMSCs) isolated and cultured in appropriate conditions, testing the ability of its multi-differentiation. Methods Cell Biology, Stem cell culture, molecular biology, stem cell tissue engineering and other methods of hMSCs in isolated, cultured and detected multiple differentiation potential. hMSCs proliferation results in a certain range (2.5% ~ 20%) with the increase of serum concentration, 20% of the serum concentration of the most appropriate and the proliferation activity in a certain Planting density range (1 ~ 8 104/ml) with increased number of cells increased planting, planting density of 8 104/ml cells, the cell proliferation activity of the highest. The isolated, cultured hMSCs is pure and can be Long-term passage, amplification and stability of the cell. hMSCs in adipogenic medium in the formation of fat cells, the Sudan staining cytoplasm of the fat cells stained orange. in the medium into the cartilage cells to form cartilage, Alcian blue staining structure of cartilage nodules stained dark blue. osteogenic medium in the successful formation of bone cells, Von Kossa staining showed mineralized nodule formation in black. The cells in the induction of a specific culture conditions, with the fat cells, cartilage cells, osteoblasts differentiation, in line with hMSCs determine the “gold standard.” conclusion of the isolation, culture of hMSCs is pure, long passage, amplification and maintain the undifferentiated state, stable cell, and its in vitro conditions may apply to the fat cells, cartilage cells, osteoblast differentiation, is a kind of multi-differentiation potential of cells. [Keywords:] Mesenchymal stem cells; bone marrow; multiple differentiation Human bone